利用高分辨率液相色谱串联质谱方法分析M1、M2型巨噬细胞的甘油三酯脂质谱组成

Using high-resolution liquid chromatography tandem mass spectrometry method to analyze the triglyceride profile in M1 and M2 macrophages

目的通过高分辨率液相色谱串联质谱方法分析M1、M2型巨噬细胞的甘油三酯脂质谱组成,揭示脂质代谢尤其是甘油三酯在巨噬细胞极化过程中的作用。方法采用小鼠骨髓细胞来源的巨噬细胞极化得到M1、M2型巨噬细胞,实时定量聚合酶链反应方法检测其极化标志基因的表达,使用高分辨率液相色谱串联质谱方法检测M0、M1、M2型巨噬细胞的甘油三酯脂质谱,对比分析不同极化状态巨噬细胞之间的差异。结果共检测到61种甘油三酯脂质分子。甘油三酯总体水平在M1型巨噬细胞中显著上调,而在M2型巨噬细胞中显著下调。在M0、M1、M2型巨噬细胞中检测到的甘油三酯分子绝大多数是长链甘油三酯。长链不饱和甘油三酯在M1型巨噬细胞中显著上调,在M2型巨噬细胞中显著下调。结论巨噬细胞极化导致其甘油三酯脂质谱组成显著改变。

Objective To analyzed the lipid profiles of M1 and M2 macrophages via high-resolution liquid chromatography tandem mass spectrometry(LC-MS/MS)so as to reveal the important role played by lipid metabolism,especially triglyceride,in macrophage polarization.Methods Polarized M1 and M2 macrophages were generated from bone marrow-derived macrophages,and assessed the expression levels of marker genes in polarized macrophages by quantitative polymerase chain reaction,high-resolution LC-MS/MS was used to analyze the triglyceride profile of polarized macrophages and determine the difference between polarized macrophages.Results 61 triglyceride species were detected in polarized macrophages.The total triglyceride level was upregulated in M1 macrophages,but downregulated in M2 macrophages.Most of the detected triglycerides contain long-chain fatty acids.Triglycerides with long-chain polyunsaturated fatty acids were upregulated in M1 macrophages,but downregulated in M2 macrophages.Conclusion Macrophage polarization lead to a significant change of the triglyceride profile.