蒙药十一味丁香散对哮喘小鼠干预作用的实验研究

Experimental Study on the Interventional Effect of Mongolian Medicine Eleven Flavors Dingxiang Powder on Asthma Mice

目的:探讨蒙药十一味丁香散对哮喘小鼠气道高反应、血清IgE及肺组织病理学改变的影响。方法:40只雄性BALB/c小鼠被随机分为4组,每组10只,分别为空白组、模型组、蒙药一十味丁香散组、布地奈德组。采用屋尘螨滴鼻法复制支气管哮喘模型。测定醋甲胆碱激发下的小鼠基础增强呼气间歇(Penh)值,检测各组哮喘小鼠气道高反应性,HE染色观察各组小鼠肺组织病理形态学变化,ELISA法检测血清中IgE浓度。结果:与模型组比较,蒙药十一味丁香散组气道高反应降低,血清中IgE浓度下降,肺组织损伤明显减轻。结论:蒙药十一味丁香散可降低哮喘小鼠的气道高反应和血清中IgE浓度,减轻肺组织病理损伤。

Objective: To explore the effects of Mongolian medicine Shiyiwei Dingxiang San on airway hyperresponsiveness,serum IgE, and pathological changes in lung tissue in asthmatic mice. Method: 40 male BALB/c mice were randomly divided into 4 groups, with 10 mice in each group, namely blank group, model group, Mongolian medicine Yishiwei Dingxiang powder group,and budesonide group. Using the house dust mite nasal drip method to replicate a model of bronchial asthma. Measure the basal enhanced expiratory interval(Penh) value of mice stimulated by acetylcholine, detect airway hyperresponsiveness in each group of asthma mice, observe pathological morphological changes in lung tissue of each group of mice with HE staining, and detect IgE concentration in serum using ELISA method. Result: Compared with the model group, the Mongolian medicine Eleven Flavors Dingxiang Powder group showed a decrease in airway hyperresponsiveness, a decrease in serum IgE concentration, and a significant reduction in lung tissue damage. Conclusion: Mongolian medicine Eleven Flavors Dingxiang Powder can reduce airway hyperresponsiveness and serum IgE concentration in asthmatic mice, and alleviate pathological damage to lung tissue.