牙周炎患者病损组织中Foxp3 mRNA、IL-34 mRNA和miR-146a-5p表达水平与牙周指标的关系

Relationship between the expression levels of Foxp3 mRNA,IL-34 mRNA,miR-146a-5p and periodontal indexes in patients with periodontitis

目的:分析牙周炎患者炎症病损组织中叉头翼螺旋转录分子(transcription factor forkhead box protein 3,Foxp3)mRNA、白介素-34(interleukin-34,IL-34)mRNA、微小RNA(micro RNA,miRNA)-146a-5p的表达水平与牙周指标之间的相关性。方法:将2021年4月~2023年4月我院收治的牙周炎患者118例纳入研究组[轻度(n=51)、中度(n=42)、重度(n=25)],另择同期阻生齿拔除患者125例纳入对照组。比较两组牙周组织中Foxp3 mRNA、IL-34 mRNA、miR-146a-5p的表达水平,不同牙周炎症程度的患者实验室指标及牙周指标,通过Pearson相关性分析法分析牙周炎患者病损组织中Foxp3 mRNA、IL-34 mRNA、miR-146a-5p与牙周指标的相关性。结果:研究组病损组织中Foxp3 mRNA、IL-34 mRNA、miR-146a-5p表达量高于对照组(P<0.05)。轻度组、中度组、重度组牙周炎组织中Foxp3 mRNA、IL-34 mRNA、miR-146a-5p表达量,菌斑指数(plaque index, PLI)、牙龈指数(gingival index, GI)、牙周探诊深度(probing depth, PD)呈升高趋势,组间比较差异均具有统计学意义(P<0.05)。牙周炎患者组织中Foxp3 mRNA、IL-34 mRNA、miR-146a-5p与PD、PLI、GI均呈正相关(P<0.05)。结论:牙周炎患者组织中Foxp3 mRNA、IL-34 mRNA、miR-146a-5p表达量与病情进展、牙周指标密切相关,可作为牙周炎防治及疗效评估的潜在靶分子。

Objective:To analyze the relationship between the expression levels of transcription factor forkhead box protein 3(Foxp3)mRNA,interleukin-34(IL-34)mRNA,micro RNA(miRNA)-146a-5p and periodontal indexes in patients with periodontitis.Methods:A total of 118 patients with periodontitis admitted to our hospital from April 2021 to April 2023 were included in the study group[mild(n=51),moderate(n=42),severe(n=25)],and 125 patients with impacted tooth extraction were included in the control group during the same period.The levels of Foxp3 mRNA,IL-34 mRNA,miR-146a-5p, laboratory and periodontal indexes of patients with different periodontal inflammation were compared between the study group and the control group.The correlation between Foxp3 mRNA,IL-34 mRNA,miR-146a-5p and periodontal indexes in periodontitis patients was analyzed by Pearson correlation analysis.Results:The expressions of Foxp3 mRNA,IL-34 mRNA and miR-146a-5p in the study group were higher than those in the control group(P<0.05).The expression levels of Foxp3 mRNA,IL-34 mRNA,miR-146a-5p, plaque index(PLI),gingival index(GI)and periodontal probing depth(PD)showed an increasing trend in the mild, the moderate and the severe groups were statistically significant(P<0.05).Tissue of periodontitis of Foxp3 mRNA,IL-34 mRNA,miR-146a-5p in patients with periodontitis were positively correlated with PD,PLI,GI(P<0.05).Conclusion:The expression levels of Foxp3 mRNA,IL-34 mRNA and miR-146a-5p in patients with periodontitis were closely related to disease progression and periodontal indexes, and could be used as potential target molecules for the prevention and treatment of periodontitis.