谷子Glycosyl hydrolases family 17鉴定及在逆境胁迫下的表达分析

Identification of Glycosyl hydrolases family 17 in Foxtail Millet and Expression Analysis under Adversity Stress

糖苷水解酶17(GH17)在调节植物生长发育、响应生物和非生物胁迫过程中发挥重要作用。为深入鉴定谷子中的GH17s基因,并初步探明其在谷子生长发育以及逆境胁迫响应方面的功能,本试验对58个SiGH17s的生物信息学和谷子响应禾生指梗霉(Sclerospora graminicola)侵染、低氮、低磷以及干旱胁迫的表达模式进行了分析。结果显示,58个SiGH17s编码的蛋白长度存在差异,与水稻、拟南芥具有较高结构和功能的相似性;该家族启动子中存在多种与抗病相关的顺式作用元件。谷子中9对共线性片段重复基因受到纯化选择;6个基因在谷子受到低氮、低磷、干旱胁迫以及禾生指梗霉侵染后均不同程度差异表达。结合实时荧光定量PCR(qRT-PCR)验证,发现6个基因中的4个差异表达基因(SiGH17_4、SiGH17_20、SiGH17_26、SiGH17_38)在谷子生长发育和逆境胁迫应答中均受到不同程度重调控。本研究结果为深入解析SiGH17s的功能提供了理论依据。

Glycosyl hydrolases family 17(GH17)plays an important role in regulating plant growth and development,responding to biotic and abiotic stresses.In order to further identify GH17 genes in foxtail millet and preliminarily investigate its functions in foxtail millet growth and development as well as stress response,in this study,the bioinformatics of 58 SiGH17s and the expression patterns of foxtail millet in response to S.graminicola infection,low nitrogen,low phosphorus and drought stress were analyzed.The results showed that the length of 58 SiGH17s encoded proteins was different,which had high structural and functional similarity with Oryza sativa and Arabidopsis thaliana.There are many cis-acting elements related to disease resistance in the promoter of this gene family.Collinear segment repeats of 9 pairs in foxtail millet were purified and selected.Totally 6 genes were differentially expressed to varying degrees after the foxtail millet was exposed to low nitrogen,low phosphorus,drought stress and the infection of S.graminicola.Combined with qRT-PCR,it was found that 4 differentially expressed genes(SiGH17_4,SiGH17_20,SiGH17_26,SiGH17_38)among the 6 genes were reregulated to varying degrees in foxtail millet growth and development and stress response.The results of this study provide theoretical basis for further analysis of SiGH17s function.