香樟bZIP基因家族鉴定及表达分析

Identification and functional analysis of the bZIPgene family of Cinnamomum camphora

碱性亮氨酸拉链(bZIP)基因能响应多种非生物胁迫,为探究香樟(Cinnamomum camphora)bZIP基因是否具有调控非生物胁迫的能力,对其进行全基因组鉴定和系统进化分析,并分析其保守基序、基因结构、保守结构域、理化性质及顺式作用元件。结果表明:香樟中共有56个bZIP基因(CcbZIP),分为10个亚族。同一亚家族的基因保守基序相似,而不同亚家族的基因保守基序存在较大差异。56个CcbZIP基因不均匀地分布在12条染色体上,且存在片段复制事件,香樟与毛果杨(Populus trichocarpa)的共线基因最多。CcbZIP基因家族含有光响应元件最多,还含有大量干旱、低温、厌氧等非生物胁迫响应元件和激素类响应元件。表达量分析显示,土壤肥力差的环境下多数CcbZIP基因在枝和叶中高表达,施肥后土壤肥力增强而基因表达量降低。

Basic Leucine zipper(bZIP)gene can respond to a variety of abiotic stress.To explore Cinnamomum camphora whether bZIPgene has the ability to regulate abiotic stress,the whole genome identification and phylogenetic analysis were carried out.The results showed that there were a total of 56 bZIPgenes(CcbZIP)in Cinnamomum camphora,divided into 10subfamilies.The conserved motifs,gene structure,conserved domains,and physicochemical properties indicated that the conserved motifs of genes in the same subfamily were similar,while there were significant differences in the conserved motifs of genes in different subfamilies.56 CcbZIPgenes were unevenly distributed on 12chromosomes,and there were fragment replication events,Cinnamomum camphora and Populus trichocarpa had the most collinear genes.The cis-regulatory element showed that the CcbZIPgene family contained the most light response element,as well as a large number of abiotic stress response element such as drought,low temperature,anaerobic and hormone response element.Expression analysis showed that most CcbZIPgenes were highly expressed in branches and leaves under poor soil conditions,and the expression level of soil fertility enhancing genes decreased after fertilization.