CNPY3通过EMT信号通路促进结肠癌细胞增殖和侵袭

CNPY3 promotes the proliferation and invasion of colorectal cancer cells through EMT signaling pathway

目的:探讨冠层FGF信号调节因子3(canopy FGF signal regulator 3,CNPY3)对结肠癌细胞增殖和侵袭的影响及其分子机制。方法:通过TCGA数据库分析和Western blot法检测CNPY3在结肠癌组织中的表达。将shControl,shCNPY3-01和shCNPY3-02慢病毒转染至结肠癌细胞株HCT116和SW480中,采用CCK8法和克隆形成实验检测CNPY3对结肠癌细胞增殖的影响,Transwell实验检测CNPY3对结肠癌细胞侵袭的影响,Western blot法测定结肠癌细胞中上皮-间充质转化(epithelial-mesenchymal transition,EMT)信号通路钙粘蛋白E(E-cadherin)、钙粘蛋白N(N-cadherin)及波形蛋白(vimentin)的表达。结果:TCGA数据库分析显示CNPY3在结肠癌组织表达水平明显高于癌旁正常结肠组织(P<0.01)。Western blot检测结果显示,手术切除的结肠癌标本中CNPY3表达水平明显高于癌旁正常结肠组织标本(P<0.01)。CCK8法和克隆形成实验结果显示,敲低结肠癌细胞CNPY3基因后,细胞增殖能力显著降低(P<0.01);Transwell实验结果显示,敲低结肠癌细胞CNPY3基因后,细胞侵袭能力显著降低(P<0.01)。Western blot检测结果显示,敲低CNPY3基因后结肠癌细胞E-cadherin表达增加,N-cadherin和vimentin表达降低(P<0.01)。结论:CNPY3在结肠癌组织中高表达,CNPY3可能通过EMT信号通路调控结肠癌细胞的增殖和侵袭。

Objective:To investigate the effect of canopy FGF signal regulator 3(CNPY3)on the proliferation and invasion of colorectal cancer cells and its molecular mechanism.Methods:The expression of CNPY3 in colorectal cancer tissues was analyzed by the TCGA database and Western blot method.The effect of CNPY3 on the proliferation was detected by CCK8 and colony formation.The effect of CNPY3 on the invasion of colorectal cancer cells was detected by Transwell assay.The expression of E-cadherin,N-cadherin,and vimentin proteins related to the epithelial-mesenchymal transition(EMT)signaling pathway in colorectal cancer cells was determined by Western blot.Results:TCGA database analysis showed that the expression level of CNPY3 in colorectal cancer tissues was significantly higher than that in the adjacent normal colorectal tissues(P<0.01).The results of Western blot showed that the expression level of CNPY3 in the resected colorectal cancer specimens was significantly higher than that in the adjacent normal colorectal tissue specimens(P<0.01).CCK8 assay and colony formation assay showed that CNPY3 knockdown significantly suppressed the proliferation of colorectal cancer cells(P<0.01).Transwell assay showed that CNPY3 knockdown significantly inhibited the invasion ability of colorectal cancer cells(P<0.01).Western blot results showed that after CNPY3 knockdown,the expression of E-cadherin in colorectal cancer cells increased,and the expression of N-cadherin and vimentin decreased(P<0.01).Conclusions:CNPY3 is highly expressed in colorectal cancer tissues and may regulate the proliferation and invasion of colorectal cancer cells through EMT signaling pathway.