黄芪甲苷对慢性阻塞性肺疾病大鼠氧化应激和气道重构的影响

Effects of Astragaloside on Oxidative Stress and Airway Remodeling in Rats with Chronic Obstructive Pulmonary Disease

目的 探讨黄芪甲苷对慢性阻塞性肺疾病(Chronic Obstructive Pulmonary Disease,COPD)大鼠氧化应激和气道重构的影响。方法 选取70只SD大鼠,采用随机数字表法分为正常组、模型组、N-乙酰半胱氨酸组、地塞米松组、黄芪甲苷低中高剂量组,各10只。正常组大鼠不予任何处理。以烟熏的方法制备COPD大鼠模型,治疗组分别给予N-乙酰半胱氨酸、地塞米松、黄芪甲苷干预。24周后,检测大鼠肺功能、氧化应激与气道重构相关指标的变化。结果 与模型组相比,黄芪甲苷高剂量组大鼠肺功能相关指标、血清细胞因子、肺泡灌洗液细胞因子、氧化/抗氧化酶指标均显著优于模型组,差异有统计学意义(P均<0.05)。结论 黄芪甲苷可能通过清除氧自由基,减少气道炎症及氧化应激水平,改善或延缓COPD气道重塑的发生。

Objective To investigate the effects of astragaloside on oxidative stress and airway remodeling in chronic obstructive pulmonary disease(COPD) rats.Methods Seventy SD rats were selected and divided into normal group,model group,N-acetylcysteine group,dexamethasone group,and low,medium,and high dose astragaloside group by randomized table method,10 rats in each group.The rats in the normal group were not treated in any way.The COPD rat model was prepared by smoking,and the treatment group was given N-acetylcysteine,dexamethasone,and astragaloside interventions,respectively.24 weeks later,changes in lung function,oxidative stress and airway remodelingrelated indexes were detected in the rats.Results Compared with the model group,lung function-related indexes,serum cytokines,alveolar lavage cytokines,and oxidative/antioxidant enzymes indexes of rats in the high dose astragaloside group were significantly better than those of the model group,and the differences were statistically significant(all P<0.05).Conclusion Astragaloside can improve or delay the occurrence of airway remodeling in COPD by scavenging oxygen free radicals and reducing airway inflammation and oxidative stress levels.