HPV-DNA与E6/E7mRNA联合检测对宫颈癌筛查的价值分析

Value Analysis of Combined Detection of HPV-DNA and E6/E7mRNA in Cervical Cancer Screening

目的探讨HPV-DNA与E6/E7mRNA联合检测对宫颈癌筛查的价值。方法选取本院2021年1月—2022年1月收治的可疑宫颈病变早筛患者1200例作为研究对象。分别使用宫颈液基细胞学(TCT)检查、人乳头瘤病毒(HPV)E6/E7mRNA检测、第二代杂交捕获实验(HC-2)HPV-DNA检测以及阴道镜下病理活检手段对宫颈癌进行筛查与诊断。分析HPV E6/E7mRNA检测以及HPV-DNA检测在宫颈早期癌变中的诊断价值。结果总体HPV E6E7mRNA阳性率42.33%,HPV E6/E7mRNA定量分析(经对数转换)平均(2.25±0.39)。总体HPV-DNA阳性率57.42%,HPV-DNA定量分析平均(489.53±102.43)。不同病理结果患者的HPV E6/E7mRNA以及DNA定性和定量分析结果,差异均有统计学意义(P<0.05)。ROC结果显示两者联合检测的曲线下面积0.801,约登指数0.618,高于单独检测。结论HPV-DNA与E6/E7mRNA在正常宫颈组织及炎性宫颈组织中的表达较低,而在CIN组织中的表达显著上升。HPV-DNA与E6/E7mRNA联合检测对宫颈癌筛查的价值明确。

Objective To explore value of combined detection of HPV-DNA and E6/E7mRNA in cervical cancer screening.Methods The paper chose 1200 suspected early screening patients with cervical lesions in our hospital from January 2021 to January 2022 as research subjects,screened and diagnosed cervical cancer with cervical liquid based cytology(TCT),human papillomavirus(HPV)E6/E7mRNA detection,second-generation hybrid capture assay(HC-2)HPV-DNA detection,and pathological biopsy under colposcopy.Diagnostic value of HPV E6/E7mRNA detection and HPVDNA detection for early cervical cancer was analyzed.Results Overall positive rate of HPV E6E7 mRNA was 42.33%,quantitative analysis of HPV E6/E7mRNA(logarithmic conversion)averaged(2.25±0.39).Overall HPV-DNA positivity rate was 57.42%,and average HPV-DNA quantitative analysis was(489.53±102.43).Difference in HPV E6/E7mRNA and DNA qualitative and quantitative analysis of patients with different pathological outcomes was statistically significant(P<0.05).ROC results showed area under combined detection curve was 0.801,with Yoden index of 0.618,which was higher than single detection.Conclusion Expression of HPV-DNA and E6/E7mRNA is lower in normal cervical tissue and inflammatory cervical tissue,but significantly higher in CIN tissue.Combined detection of HPV-DNA and E6/E7mRNA has clear value in cervical cancer screening.