组蛋白去乙酰化酶5在胃癌中的表达及与肿瘤免疫微环境的关系

Expression of histone deacetylase 5 in gastric cancer and its relationship with tumor immune microenvironment

目的探讨组蛋白去乙酰化酶5(HDAC5)在胃癌中的表达及与肿瘤免疫微环境的关系。方法回顾性分析本院2021年2月—2023年12月诊治的120例胃癌患者的临床资料。采用免疫组化分析法检测患者胃癌组织中HDAC5的表达水平,并将患者分为HDAC5高表达组与低表达组。采用单因素分析、多因素Logistic回归分析及受试者工作特征(ROC)曲线探讨影响HDAC5表达的相关因素;检测2组组织中外周血调节性T细胞(Treg)、肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、降钙素原(PCT)、超敏C反应蛋白(hs-CRP)、免疫球蛋白A(IgA)、免疫球蛋白M(IgM)、免疫球蛋白G(IgG)水平;采用Pearson相关分析验证HDAC5在胃癌中的表达水平与肿瘤免疫微环境的相关性。结果单因素分析结果显示,2组肿瘤直径、Borrmann分型、淋巴结转移情况的差异有统计学意义(P<0.05)。多因素Logistic回归分析结果显示,肿瘤直径、Borrmann分型以及淋巴结转移情况是HDAC5在胃癌中表达水平的独立影响因素(P<0.05)。ROC曲线分析结果显示,肿瘤直径、Borrmann分型以及淋巴结转移情况的曲线下面积(AUC)分别为0.608、0.593、0.614。Pearson相关分析结果显示,胃癌患者的HDAC5表达水平与Treg含量、炎症介质指标均呈正相关(P<0.05),与IgA、IgM、IgG呈负相关(P<0.05)。结论在胃癌患者中,HDAC5的表达水平与肿瘤直径、Borrmann分型以及淋巴结转移情况相关,高水平的HDAC5会直接影响胃癌的肿瘤免疫微环境。

Objective To investigate the expression of histone deacetylase 5(HDAC5)in gastric cancer and its relationship with tumor immune microenvironment.Methods Clinical materials of 120 patients with gastric cancer diagnosed and treated in the hospital from February 2021 to December 2023 were retrospectively analyzed.Immunohistochemical analysis was used to detect the expression level of HDAC5 in gastric cancer tissues,and the patients were divided into HDAC5 high-expression group and low-expression group.Univariate analysis,Logistic regression analysis,and receiver operating characteristic(ROC)curve were used to explore the related factors affecting HDAC5 expression.The levels of regulatory T cells(Treg),tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6),procalcitonin(PCT),hypersensitive C-reactive protein(hs-CRP),immunoglobulin A(IgA),immunoglobulin M(IgM),and immunoglobulin G(IgG)in both groups were detected.Pearson correlation analysis was used to verify the correlation between HDAC5 expression level and tumor immune microenvironment in gastric cancer.Results Univariate analysis showed that there were significant differences in tumor diameter,Borrmann classification and lymph node metastasis between the two groups(P<0.05).Logistic regression analysis showed that tumor diameter,Borrmann classification and lymph node metastasis were independent risk factors affecting HDAC5 expression in gastric cancer(P<0.05).ROC curve analysis showed that the values of area under the curve(AUC)for tumor diameter,Borrmann classification and lymph node metastasis were 0.608,0.593 and 0.614,respectively.Pearson correlation analysis showed that HDAC5 expression level in gastric cancer patients was positively correlated with Treg content and inflammatory mediator indexes(P<0.05),and negatively correlated with IgA,IgM and IgG(P<0.05).Conclusion In patients with gastric cancer,the expression level of HDAC5 is related to tumor diameter,Borrmann classification and lymph node metastasis.High-level HDAC5 can directly affect the tumor immune microenvironment of gastric cancer.