ARTP诱变选育高产腺苷菌株及发酵条件优化

Breeding of High-Producing Adenosine Strains by ARTP Mutation and Optimization of Fermentation Conditions

为获得高产腺苷菌株,实现降本增效,该研究基于一株腺苷产生菌枯草芽孢杆菌SB-37,对其进行ARTP诱变选育,采用单因素实验和响应面方法优化菌株发酵条件。结果表明,最佳处理时间为30 s,菌株诱变致死率为98.75%。比对初始菌株腺苷产量,获得8株高产腺苷突变株,其中菌株Q40-2-37腺苷产量最高,为6.20 g/L,是出发菌株的2.58倍。该菌株最适发酵条件为葡萄糖99 g/L、蛋白胨25 g/L、pH 7.04、接种量8.03%、发酵时间72 h,此条件下,腺苷产量为6.92 g/L。该研究结果为高产腺苷菌株的诱变选育和发酵条件的优化提供了技术支撑。

In order to obtain high-producing adenosine strains and realize cost reduction and efficiency enhancement,a strain of adenosine-producing bacterium,Bacillus subtilis SB-37,is breeded by ARTP mutation,and single factor experiment and response surface method are used to optimize the fermentation conditions of the strain.The results show that the optimal treatment time is 30 s and the mutation fatality rate is 98.75%.Compared with the adenosine yield of the original strain,8 high-producing adenosine mutant strains are obtained,and strain Q40-2-37 has the highest adenosine yield of 6.20 g/L,which is 2.58 times that of the original strain.The optimal fermentation conditions of the strain are glucose of 99 g/L,peptone of 25 g/L,pH of 7.04,inoculation amount of 8.03%and fermentation time of 72 h.Under these conditions,the adenosine yield is 6.92 g/L.The results of this study have provided technical support for the mutation and breeding of high-producing adenosine strains and optimization of fermentation conditions.