磁刺激对巩膜成纤维细胞生长的影响

Effect of Magnetic Stimulation on the Growth of Scleral Fibroblasts

近视的发病机制之一是巩膜因素介导,巩膜成纤维细胞是眼球壁最外层组织巩膜的重要组成部分。通过共价沉淀法制备磁/石墨烯(MNP/G)复合纳米材料,将其导入巩膜成纤维细胞进行磁刺激,观察磁刺激对细胞生长的影响,用CCK-8法检测巩膜成纤维细胞的增值状态,采用倒置相差显微镜观察细胞的生长状态,最后通过测定羟脯氨酸的浓度推测巩膜组织的韧性情况。研究结果表明:磁刺激能够像正常状态一样促进细胞生长,还可以促进胶原蛋白合成。磁刺激可能成为促进巩膜韧性增强的一种手段,为近视的预防和矫正提供了新的方法和思路。

One of the mechanisms of myopia is mediated by scleral factors,and sleral fibroblasts are an important component of the outermost tissue of the eyeball.This study prepared magnetic/graphene(MNP/G)composite nanomaterials using covalent precipitation method and introduced them into scleral fibroblasts for magnetic stimulation.The effect of magnetic stimulation on cell growth was observed,and the proliferation status of scleral fibroblasts was detected using CCK-8 method.The growth status of cells was observed using an inverted phase contrast microscope,and the toughness of scleral tissue was inferred by measuring the concentration of hydroxyproline.The results indicated that magnetic stimulation can promote cell growth without any difference from the normal state as well as collagen synthesis.Magnetic stimulation may be a method to increase scleral toughness,and be expected to provide new methods and ideas in the prevention and correction of myopia.