花青素颜色指示标签在羊肉新鲜度无损检测中的应用

Application of Anthocyanin Color Indicator Label in Non-destructive Testing of Mutton Freshness

目的研发一种能够可视化、无损检测羊肉新鲜度的颜色指示标签,便于消费者更加直观、准确地判断羊肉新鲜度。方法本研究以聚乳酸为基材,玫瑰花青素为指示剂,采用静电纺丝技术制备了颜色指示标签(PLA/Ac)。通过扫描电镜、傅里叶红外光谱、氨敏感性、湿度稳定性、温度稳定性和可重复性对PLA/Ac颜色指示标签进行表征,并验证PLA/Ac颜色指示标签对羊肉新鲜度的检测效果。结果表征结果显示,PLA/Ac颜色指示标签具有均匀分布的纺锤体结构,平均纤维直径为541.96 nm,并成功负载玫瑰花青素。此外,PLA/Ac颜色指示标签还具有良好的氨敏感性、湿度稳定性、温度稳定性和可重复性。应用结果显示,羊肉在25℃贮藏期间香气轮廓发生变化,酸味逐渐下降,苦味逐渐上升,咸味和鲜味则呈现出先升后降的变化趋势。当羊肉在25℃贮藏48 h后,总挥发性盐基氮值和菌落总数超标,此时PLA/Ac颜色指示标签的颜色由粉色变为灰色。结论PLA/Ac颜色指示标签在可视化、无损检测羊肉新鲜度领域具有良好的应用前景,对保障消费者健康和促进行业发展具有积极作用。

The work aims to develop a color indicator label that can visually and non-destructively test the freshness of mutton so that consumers can determine the freshness of mutton more intuitively and accurately.In this study,an electrostatic spinning technique was used to prepare a color indicator label(PLA/Ac)with polylactic acid as the base material and rose anthocyanin as the indicator.The PLA/Ac,color indicator label,was characterized by scanning electron microscopy(SEM),Fourier infrared spectroscopy(FTIR),ammonia sensitivity,humidity stability,temperature stability,and reproducibility.The PLA/Ac color indicator label,was verified for the detection of freshness in mutton.Characterization results showed that PLA/Ac color indicator label had a uniformly distributed spindle structure with an average fiber diameter of 541.96 nm and was successfully loaded with rose anthocyanins.In addition,the PLA/Ac color indicator label had good ammonia sensitivity,humidity stability,temperature stability,and reproducibility.The application results showed that the aroma profile of mutton changed during storage at 25℃,with sourness gradually decreasing,bitterness increasing,and salty and fresh flavors increasing and then decreasing.After the mutton was stored at 25℃for 48 hours,the total volatile saline nitrogen value and total colony count exceeded the standard.At this time,the color of the PLA/Ac color indicator label changed from pink to gray.The PLA/Ac color indicator label has a good potential for application in the field of visualization and non-destructive testing of mutton freshness,which has a positive effect on safeguarding consumer health and promoting the development of the industry.