复合蛋白酶水解菲律宾蛤蜊及产物的生物学活性研究

Biological Activity of Composite Protease Hydrolyzed Ruditapes philippinarum and Its Products

蛋白酶水解蛤蜊蛋白制备生物活性肽可以提高产品的附加值。以菲律宾蛤蜊(Ruditapes philippinarum)为原料,采用复合酶法,以水解度和抗氧化活性为指标,优化蛋白酶的组合和酶解条件。结果表明,当木瓜-菠萝-中性蛋白酶以2:1:1组合时,在加酶量0.1%、底物浓度5%、pH7、酶解温度40℃和水解时间3 h的条件下,蛤蜊蛋白的水解度达到78.17%。水解液超滤后,分子质量小于3 ku组分的1,1-二苯基-2-三硝基苯肼(1,1-Diphenyl-2-picrylhydrazyl,DPPH)自由基清除率为86.92%,羟基自由基清除率为89.48%,总还原力为0.50。复合蛋白酶可以显著提高蛤蜊蛋白的水解度和生物学活性,研究结果为蛤蜊的精深加工提供了实验依据。

Protease hydrolysis of clam protein to prepare bioactive peptides can increase the products added value.This study used Ruditapes philippinarum as raw material,and selected composite enzymes to screen the combination of proteases and enzymatic hydrolysis conditions based on hydrolysis degree and antioxidant activity.The results showed that the degree of hydrolysis of clam protein reached 78.17%when papain,bromelain,and neutral protease were combined in a 2:1:1 ratio,under the conditions of enzyme addition of 0.1%,substrate concentration of 5%,pH 7,temperature of 40℃,and hydrolysis 3 h.After ultrafiltration of the hydrolysate,the fraction with a molecular weight of less than 3 ku had 1,1-diphenyl-2-picrylhydrazyl(DPPH)free radical scavenging rate of 86.92%,hydroxyl free radical scavenging rate of 89.48%,and total reducing power of 0.50.Composite proteases could significantly improve the hydrolysis degree and biological activity of clam protein and the results provide experimental basis for the deep application clams.