大豆多肽与玉木耳多糖联合对小鼠抗疲劳作用及肠道微生物影响的研究

Effects of Soybean Peptides and Auricularia cornea Polysaccharides on Anti Fatigue and Gut Microbiota in Mice

文章探讨了大豆多肽(Soybean peptides,SP)和玉木耳多糖(Auricularia cornea polysaccharides,AP)联合使用对小鼠抗疲劳作用以及肠道微生物的影响。将小鼠随机分为4组,进行为期4周的饮食干预,分别为对照组(Control group,CK组)、SP组、AP组和SP-AP组,通过测定血清生化指标、抗氧化指标和能量代谢相关指标以分析SP和AP联合使用的抗疲劳作用机制。结果表明:SP和AP联合使用显著提高了小鼠力竭游泳时间,与CK组小鼠相比,SP-AP联合使用显著降低了力竭游泳小鼠血清中尿素氮(Blood urea nitrogen,BUN)、乳酸(Lactic acid,LA)和肿瘤坏死因子α(Tumor necrosis factor,TNF-α)水平,显著提高了白介素6(Interleukin-6,IL-6)、肝糖原和肌糖原水平。SP-AP联合使用通过提高小鼠肝脏和肌肉超氧化物歧化酶(Superoxide dismutase,SOD)、过氧化物酶(Catalase,CAT)和谷胱甘肽过氧化物酶(Glutathione peroxidase,GSH-Px)的活性和降低丙二醛(Malondialdehyde,MDA)水平来改善运动疲劳。此外,SP-AP联合组显著提高了小鼠肝脏和肌肉中Ca^(2+)Mg^(2+)-ATP酶(Ca^(2+)Mg^(2+)-ATPase)、琥珀酸脱氢酶(Succinate dehydrogenase,SDH)和丙酮酸激酶(Pyruvate kinase,PK)活力。此外,SP和/或AP的使用也显著改变了小鼠肠道微生物组成。综上所述,SP-AP联合使用可通过降低血清中BUN和LA积累,提高机体抗氧化能力,调节机体能量代谢过程来改善小鼠运动疲劳,这或许与小鼠肠道微生物组成改变也有一定关联。研究结果为抗疲劳功能性食品的开发提供了一种新的思路。

The study investigated the combined effects of soybean peptides (SP) and Auricularia cornea polysaccharides (AP) on anti fatigue and gut microbiota in mice.The mice were randomly divided into four groups for a 4-week dietary intervention,namely the control group (CK group),SP group,AP group,and SP-AP group.The combined effects of SP and AP on the anti fatigue mechanism were analyzed by measuring serum biochemical indicators,antioxidant indicators,and energy metabolism related indicators.The results showed that the combined using of SP and AP significantly improved the exhaustion swimming time of mice.Compared with the CK group mice,the combined using of SP-AP significantly reduced the blood serum urea nitrogen (BUN),lactic acid (LA),and tumor necrosis factor (TNF-ɑ) in exhausted swimming mice,significantly increased the levels of interleukin-6 (IL-6),liver glycogen,and muscle glycogen.The combined use of SP-AP improves exercise fatigue by increasing the activity of superoxide dismutase (SOD),peroxidase (CAT),and glutathione peroxidase (GSH-Px) in the liver and muscle of mice,and reducing the level of malondialdehyde (MDA).In addition,the SP-AP combination group significantly increased the activities of Ca^(2+)Mg^(2+)-ATPase,succinate dehydrogenase (SDH),and pyruvate kinase (PK) in the liver and muscle of mice.In addition,the use of SP and/or AP significantly altered the gut microbiota composition of mice.In summary,the combined use of SP-AP can improve exercise fatigue in mice by reducing the accumulation of BUN and LA in serum,improving the body’s antioxidant capacity,and regulating the body’s energy metabolism process.This may also be related to changes in the gut microbiota composition of mice.This study provides a new approach for the development of anti fatigue functional foods.