基于仿生复合材料的纸基双模信号输出检测平台测定二苯甲酮-3

A Paper-based Dual-mode Signal Output De⁃tection Platform Incorporating Biomimetic Composites for the Detection of Benzophe⁃none-3

二苯甲酮-3(BP-3)是一种广泛使用的有机紫外线滤光剂,会对环境造成污染且对生物体具有潜在毒性,因此构建能够快速、便携式检测BP-3的方法十分必要。该文将仿生复合材料与纸芯片集成,研制了一种纸基微流控分析装置(μPAD)。仿生复合材料由纳米酶和分子印迹聚合物(MIP)组成,两者分别作为信号产生的催化剂和μPAD的识别单元。首先通过简单、温和的方式合成具有过氧化物酶活性的双金属有机凝胶(Fe/Ru-MOG)纳米酶,并通过本体聚合法将其掺杂到MIP中。Fe/Ru-MOG@MIP-μPAD可通过化学发光(CL)和比色法双信号输出对BP-3进行测定。BP-3通过与MIP特异性结合可减弱Fe/Ru-MOG对H2O2的催化能力,导致CL信号强度降低和比色信号变化。通过手机原位拍摄检测区域的照片记录两种信号,然后转换为灰度值进行量化。双信号分别在1×10^(-9)~1×10^(-7) mol/L(CL)和3×10^(-9)~0.9×10^(-7) mol/L(比色)浓度范围内线性良好,检出限分别为1.63×10^(-10) mol/L(CL)和1.64×10^(-10) mol/L(比色)。且μPAD在土壤、河水和自来水等实际样品的分析中具有良好的选择性和可靠性。作为一种便携、经济的检测工具,μPAD将在快速、现场测定BP-3方面发挥重要作用。

Benzophenone-3(BP-3)is a widely used organic ultraviolet filter.Owing to its environmental contamina⁃tion and potential toxicity to organisms,rapid and convenient detection of BP-3 is needed.Herein,a microfluidic paper-based analytical device(μPAD)has been developed by integrating biomimetic composites with paper chips.The biomimetic composite consists of nanozyme and molecularly imprinted polymer(MIP),which act as the catalyst for generating signals and the recognition unit of theμPAD,respectively.In particular,the nanozyme with peroxi⁃dase activity is a dual-metal organic gel(Fe/Ru-MOG)synthesized in a simple and gentle manner and further doped with MIP via bulk polymerization.The obtained Fe/Ru-MOG@MIP-μPAD is employed for determination of BP-3 through a double-signal outputs including chemiluminescence(CL)and colorimetry.As the specific binding of BP-3 by the MIP weakens the catalytic ability of Fe/Ru-MOG towards H2O2,CL intensities decrease together with the change of colorimetric signals.Both signals were recorded by in-situ taking photos of the detection region using a mo⁃bile phone,followed by conversion to gray values for quantification.The dual signals demonstrate a good linearity within concentration ranges of 1×10^(-9)-1×10^(-7) mol/L(CL)and 3×10^(-9)-0.9×10^(-7) mol/L(colorimetry),and detection limits as low as 1.63×10^(-10) mol/L(CL)and 1.64×10^(-10) mol/L(colorimetry).The novelμPAD exhibits admirable selec⁃tivity and reliability in analysis of real samples including soil,river water and tap water.It is fair to conclude that this platform,serving as a portable and cost-effective tool,will play an important role in fast and on-site detection of BP-3.