磷胁迫下内生真菌对宿主布顿大麦生长及生理性状的影响

Influence of Endophytic Fungi on Growth and Physiological Characters of Host Hordeum bogdanii under Phosphorus Stress

本试验以新疆温宿县种植的布顿大麦为材料,基于1/2Hoagland营养液设置3个磷浓度处理,分别为无磷组(P0)、半磷组(P1)和全磷组(P2),采用水培试验研究磷胁迫下内生真菌对宿主布顿大麦生长和生理性状的影响。结果表明,随磷胁迫的减轻,布顿大麦株高和分蘖数均显著增加,内生真菌除在全磷组(P2)显著增加分蘖数外,对株高和分蘖数无显著影响。半磷组(P1)胁迫下,内生真菌对植株地上部鲜重和干重、地下部鲜重和干重以及叶片酸性磷酸酶活性的影响显著;无磷组(P0)胁迫下内生真菌对布顿大麦叶片多酚含量的影响表现为显著增加。综上,磷胁迫下内生真菌对布顿大麦的生长具有一定的促进作用,这有助于其更好地适应低磷环境,提高对生态环境的适应性。该结论可为用内生真菌创建新种质资源育种奠定一定基础。

In this study,the hydroponic experiment was conducted using Hordeum bogdanii grown in Wensu County,Xinjiang as test material.Based on 1/2Hoagland solution,three treatments were set as nophosphorus group(P0),half-phosphorus group(P1)and full-phosphorus group(P2).The effects of endo-phytic fungi on growth and physiological characters of H.bogdanii under phosphorus stress were studied.The results showed that with phosphorus stress relieving,the plant height and tiller number of H.bogdanii increased significantly,and endophytic fungi had no significant influence to them except the increase of tiller number under P2 treatment.Under P1 treatment,endophytic fungi had significant effects on fresh and dry weight of aboveground part and underground part,and acid phosphatase activity in leaves.Under P0 treatment,endophytic fungi could significantly increase the polyphenol content in leaves of H.bogdanii.In summary,endophytic fungi had a certain promotion effect on H.bogdanii growth,which was helpful for H.bogdanii to adapt low phosphorus environment,so as increasing its ecological adaptation.The conclusion could lay a certain foundation for innovation of germplasm resources with endophytic fungi.