3种鸡源乳酸菌抑制大肠杆菌生物被膜形成的比较研究

Comparison of Three Lactic Acid Bacteria from Chickens on Inhibiting Escherichia coli Biofilm Formation

试验旨在研究鼠李糖乳杆菌、戊糖片球菌和口乳杆菌3种鸡源乳酸菌对大肠杆菌生物被膜生成的影响。利用置片法对供试菌株培养48 h,定性观察细菌生长与生物被膜的形成;利用96孔微板法定量检测4种细菌生长的OD_(590)值,评价抑制生物被膜生成的效果。结果表明,大肠杆菌培养12 h开始形成生物被膜,24 h形成典型的生物被膜,并伴有交织链接的生物被膜骨架;3种乳酸菌培养36 h均未见生物被膜骨架,OD_(590)值显示鼠李糖乳杆菌生物被膜厚度优于戊糖片球菌、口乳杆菌。与大肠杆菌共培养发现,3种乳酸菌全菌液和上清液均能显著抑制大肠杆菌生物被膜的生成,抑制效果鼠李糖乳杆菌>口乳杆菌>戊糖片球菌。结果可为探索乳酸菌拮抗大肠杆菌感染机制提供参考。

This study aimed to explore the effects of Lactobacillus rhamnosus,Pediococcus pentosaceus and Lactobacillus oris on biofilm formation of Escherichia coli.After cultured for 48 hours by using the film placement method,the bacterial growth and biofilm formation were qualitatively observed,and the OD_(590) value of the four bacterial strains were quantitatively detected by 96-well microplate method to evaluate their effects on inhibiting biofilm formation.The results showed that E.coli began to form biofilm at 12 h of culture,and the typical biofilm was formed at 24 h accompanied by interwoven and linked biofilm skeleton.No biofilm skeleton was found after 36 h of co-culture with the three species of lactic acid bacteria.The OD_(590) value showed that the biofilm thickness of L.rhamnosus was better than that of P.pentosaceus and L.oris.The co-culture results showed that the whole bacterial solution and supernatant of the three species of lactic acid bacteria could significantly inhibit the formation of E.coli biofilm,and their inhibitory effects showed L.rhamnosus>L.oris>P.pentosaceus.These results could provide references for exploring the mechanism of lactic acid bacteria antagonizing E.coli infection.