小核糖核蛋白多肽E在前列腺癌中的表达及其临床意义

Expression and clinical significance of small nuclear ribonucleo protein polypeptide E in prostate cancer

目的探讨小核糖核蛋白多肽E(SNRPE)在前列腺癌(PCa)中的表达及在前列腺癌细胞中调控功能。方法基于肿瘤基因组图谱(TCGA)数据库对目的基因SNRPE进行差异分析、生存分析和临床相关性分析;通过干扰RNA(siRNA)敲低SNRPE的表达, 探究其对前列腺癌细胞增殖、迁移和侵袭能力的影响。组间比较采用独立样本t检验。结果 SNRPE在前列腺癌样本中的表达水平明显高于正常前列腺样本(5.3±0.10比4.8±0.12, t=5.54, P<0.01)。T1+T2期SNRPE表达显著低于T3+T4期表达组(t=4.53, P<0.05)。前列腺癌淋巴结未转移病人SNRPE1表达显著低于淋巴结转移病人组(t=4.21, P<0.05)。多变量Cox回归分析表明, 年龄[风险比(HR):0.99(0.95~1.00), P>0.05]、T分期[HR:1.84(0.96~3.50), P>0.05]及N分期[HR:1.32(0.68~2.60), P>0.05]不能作为OS相关的独立预后因素, 而SNRPE表达[HR:2.00(1.20~3.50), P<0.05]、及Gleason评分[HR:3.14(1.57~6.30), P<0.01]可作为前列腺癌的预后因素。PC3中ctrl组伤口愈合面积显著高于si-SNRPE#1和si-SNRPE#2组, 组间比较差异有统计学意义[(82.30±1.21)%比(38.10±2.56)%, t=27.04, P<0.01;(82.30±1.21)%比(40.05±1.82)%, t=33.48, P<0.01]。同时前列腺癌细胞迁移侵袭实验发现, ctrl组及si-SNRPE#1和si-SNRPE#2组PC3迁移细胞分别为351.8±16. 5、189.4±10.50和176.6±23.5, 组间比较差异有统计学意义(t=14.38、10.57, P<0.01)。ctrl组及si-SNRPE#1和si-SNRPE#2组PC3侵袭细胞分别为495.6±77.9、251.2±70.8和203.4±41.2, 组间比较差异有统计学意义(t=4.02、5.74, P<0.01)。结论 SNRPE基因具有促进前列腺癌细胞的增殖、迁移和侵袭能力的功能。

Objective To investigate the expression of small nuclear ribonucleo protein polypep-tide E(SNRPE)in prostate cancer(PCa)and its regulatory function in prostate cancer cells.Methods Based on the cancer genome atlas(TCGA)database,the differential analysis,survival analysis and clini-cal correlation analysis of SNRPE gene were performed.Two groups of short interfering RNA(siRNA)were used to knock down the expression of SNRPE,and the effects on the proliferation,migration and invasion of prostate cancer cells were investigated.Independent sample t test was used for comparison between different groups.Results The expression level of SNRPE in prostate cancer samples was significantly high-er than that in normal prostate samples(5.3±0.10 vs.4.8±0.12,t=5.54,P<0.01).The expression of SNRPE in the TI+T2 stage was significantly lower than that in the T3+T4 stage expression group(t=4.53,P<0.05).The expression of SNRPE1 in patients with prostate cancer without lymph node metasta-sis was significantly lower than that in patients with lymph node metastasis(t=4.21,P<0.05).Multiva-riable Cox regression analysis showed that:age[hazard ratio(HR):0.99(0.95-1.00),P>0.05],T stage[HR:1.84(0.96-3.50),P>0.05]and N stage[HR:1.32(0.68-2.60),P>0.05]cannot be used as an independent prognostic factor related to OS,while SNRPE expression[HR:2.00(1.20-3.50),P<0.05]and Gleason score[HR:3.14(1.57-6.30),P<0.01]can be used as a prognostic factor in prostate cancer.In PC3,the wound healing area of ctrl group was significantly higher than that of the si-SNRPE#1 and si-SNRPE#2 groups,and the difference between the groups was statistically significant[(82.30±1.21)%vs.(38.10±2.56)%,t=27.04,P<0.01;(82.30±1.21)%vs.(40.05±1.82)%,t=33.48,P<0.01].Furthermore,the number of migration cells in ctrl,si-SNRPE#1 and si-SNRPE#2 PC3 groups were 351.8±16.5,189.4±10.50,176.6±23.5 respectively,and the difference was significant statistically(t=14.38,10.57,P<0.01).Moreover,the number of invasion cells in ctrl,si-SNRPE#1 and si-SNRPE#2 groups were 495.6±77.9,251.2±70.8 and 203.4±41.2 respectively,and the difference was significant statistically(t=4.02,5.74,P<0.01).Conclusion SNRPE gene can promote the proliferation,migration and invasion of prostate cancer cells.SNRPE is expected to be a new biomarker for the diagnosis and targeted therapy of prostate cancer.