升率汤对大鼠病态窦房结综合征模型窦房结电生理改变实验研究

Experimental Study on Electrophysiological Changes of Sick Sinus Syndrome Model Rats with Intervention of Shenglu Decoction

目的 观察升率汤对病态窦房结综合征模型大鼠窦房结电生理功能的干预作用,并探讨其作用机制。方法 将40只病态窦房结综合征模型大鼠随机分成模型组、升率汤高、低剂量组(简称高、低剂量组)、氨茶碱组,每组10只,另设10只大鼠为空白组。空白组和模型组给予生理盐水5 mL/d灌胃,高、低剂量组每天分别给予升率汤30.24、7.56 g/kg,氨茶碱组给予氨茶碱80 mg/kg。分别于干预前、后记录典型心电图;比较各组AA间期、窦房结恢复时间(SNRT)、校正窦房结恢复时间(CSNRT)、窦房传导时间(SACT)。结果 与空白组比较,模型组AA间期、SNRT、CSNRT、SACT显著延长(P<0.05)。与模型组比较,干预后升率汤高剂量组、氨茶碱组AA间期、SNRT、CSNRT、SACT显著缩短(P<0.05)。干预后升率汤高剂量组较氨茶碱组AA间期、SNRT、SACT显著缩短(P<0.05)。结论 高剂量升率汤能明显改善病态窦房结综合征模型大鼠窦房结电生理功能。

Objective To study the effects of Shenglu Decoction(SLD)in electrophysiological function of sinoatrial node in sick sinus model rats,and explore the mechanism.Methods Forty sick sinus model rats were randomly divided into model group,SLD high,low dose and aminophylline groups,10 in each group.The other 10 rats were selected as sham group.The sham and model groups were given intragastric administration of physiological saline as 5 mL/d,the SLD high and low dose groups were given intragastric administration of SLD as 30.24 and 7.56 g·kg^(-1).The aminophylline group was given aminophylline as 80 mg·kg^(-1).Typical electrocardiograms were recorded before and after intervention.AA interval,sinus node recovery time(SNRT),corrected sinus node recovery time(CSNRT),and sinoatrial conduction time(SACT)were compared among different groups.Results Compared with the sham group,the AA interval,SNRT,CSNRT and SACT in the model group were significantly prolonged(P<0.05).Compared with the model group,the AA interval,SNRT,CSNRT and SACT were significantly shortened in the SLD high dose and aminophylline groups after intervention(P<0.05).Compared with the aminophylline group,the AA interval,SNRT and SACT were SLD high dose group after intervention(P<0.05).Conclusion The high dose SLD could significantly improve the electrophysiological function of sinoatrial node in sick sinus model rats.