广叶绣球菌多糖-白藜芦醇纳米粒子的制备、表征及其体外抑制α-葡萄糖苷酶活性

Preparation,characterization and inhibition effects onα-glucosidase of Sparassis latifolia polysaccharides-resveratrol nanoparticles

本研究采用反溶剂沉淀法制备广叶绣球菌多糖(Sparassis latifolia polysaccharides,SLPs)-白藜芦醇(resveratrol,Res)纳米粒子(SLPs-Res NPs),对其表征后分析其体外抑制α-葡萄糖苷酶活性。试验采用水提醇沉法提取SLPs并经HZ830大孔树脂脱色除杂后,以包封率、载药量为指标优化纳米粒子制备工艺,对其表征后分析其缓释特性及对α-葡萄糖苷酶的抑制作用。结果表明,当Res与SLPs的质量比为1:32时,SLPs-Res NPs的包封率最高,为(53.73±0.76)%,载药量为(1.68±0.01)%。扫描电镜显示SLPs-Res NPs表面较为粗糙。SLPs-Res NPs平均粒径为(223.51±3.02)nm,PDI值为0.51±0.04,Zeta电位为(–21.6±1.1)mV,紫外/可见及红外光谱显示SLPs与Res发生了相互作用。SLPs-Res NPs的光热稳定性均强于游离白藜芦醇,其在模拟胃液中消化2 h后Res释放率为(9.13±0.54)%,继续在模拟肠液中消化3 h后Res释放率为(31.71±0.05)%,显著低于游离Res(P<0.05)。SLPs-Res NPs对α-葡萄糖苷酶的抑制活性显著高于Res和SLPs(P<0.05)。本研究制备得到SLPs-Res NPs,其具有较好的光热稳定性、缓释特性及抑制α-葡萄糖苷酶作用,可为SLPs和Res的进一步开发利用提供一定的理论依据。

An antisolvent precipitation method was used to prepare Sparassis latifolia polysaccharides(SLPs)-resveratrol(Res)nanoparticles(SLPs-Res NPs).After characterization,their inhibition activity to α-glucosidase was analyzed in vitro.The Sparassis latifolia polysaccharides were extracted by water extraction and alcohol precipitation method followed by purification with HZ830 macroporous resin,and then the preparation process of SLPs-Res NPs was optimized by using encapsulation efficiency and drug loading as evaluation indicators.After characterization,the sustained-release property and their inhibition effect onα-glucosidase were determined.Results revealed that when the mass ratio of Res to SLPs was 1:32,the encapsulation efficiency of SLPs-Res NPs was(53.73±0.76)%,showing the highest,and the drug loading was(1.68±0.01)%.The scanning electron microscopy showed that the surface of SLPs-Res NPs was relatively rough.The average particle size of SLPs-Res NPs was(223.51±3.02)nm.The PDI value and Zeta potential was(0.51±0.04)and(-21.6±1.1)mV,respectively.The UV/vis and Fourier transform infrared spectra exhibited that SLPs interacted with Res.The photothermal stability of SLPs-Res NPs was stronger than that of free Res.The release rate of Res from SLPs-Res NPs after 2 h of digestion in simulated gastric juice was(9.13±0.54)%,and after 3 h of digestion in simulated intestinal juice,the release rate of Res was(31.71±0.05)%,being significantly lower than that of free Res(P<0.05).The inhibitory activity of SLPs-Res NPs toα-glucosidase was significantly higher than that of Res and SLPs(P<0.05).The SLPs-Res NPs prepared in this study have good photothermal stability,sustained-release property,and inhibitory effect onα-glucosidase and are worthy of further development and utilization.