松杉灵芝发酵菌丝体中的三萜化合物

Triterpenoids identified from the fermented mycelia of Ganoderma tsugae(Polyporales,Basidiomycota)

通过中高压层析、薄层色谱层析、凝胶色谱层析等多种分离纯化技术,从松杉灵芝发酵菌丝体中制备得到15个羊毛甾烷型四环三萜化合物,通过核磁共振、质谱对化合物结构进行解析,分别鉴定为(22S,24E)-3-oxo-15α,22β-dihydroxylanosta-7,9(11),24-trien-26-oicacid(1)、(22S,24E)-3-oxo-15α-hydroxy-22β-acetoxylanosta-7,9(11),24-trien-26-oicacid(2)、(22S,24E)-3-oxo-15α,22β-diacetoxylanosta-7,9(11),24-trien-26-oicacid(3)、15-hydroxy-ganodericacidS(4)、ganodermicacidJb(5)、ganoderic acid P(6)、ganodermic acid Ja(7)、ganoderic acid X(8)、ganodermic acid TQ(9)、ganoderic acid Me(10)、ganodericacidMf(11)、ganodericacidY(12)、ganodericacidZ(13)、lanosta-7,9,(11),24-trien-3α-hydroxy-26-oic acid(14)和ganoderic acid S1(15)。以上15个化合物均为首次从松杉灵芝发酵菌丝体中分离获得。该结果丰富了灵芝属真菌发酵产物的研究,为后续的活性研究提供了物质基础。

Fifteen lanostane-type triterpenoids were isolated from the mycelia of Ganoderma tsugae through various separation and purification techniques such as medium-high pressure liquid chromatography,preparative thin layer chromatography and gel chromatography.The structures of these compounds were elucidated by nuclear magnetic resonance(NMR)and mass spectrometry(MS).They were identified as:(22S,24E)-3-oxo-15α,22β-dihydroxylanosta-7,9(11),24-trien-26-oic acid(1),(22S,24E)-3-oxo-15α-hydroxy-22β-acetoxylanosta-7,9(11),24-trien-26-oic acid(2),(22S,24E)-3-oxo-15α,22β-diacetoxylanosta-7,9(11),24-trien-26-oic acid(3),15-hydroxy-ganoderic acid S(4),ganodermic acid Jb(5),ganoderic acid P(6),ganodermic acid Ja(7),ganoderic acid X(8),ganodermic acid TQ(9),ganoderic acid Me(10),ganoderic acid Mf(11),ganoderic acid Y(12),ganoderic acid Z(13),lanosta-7,9,(11),24-trien-3α-hydroxy-26-oic acid(14)and ganoderic acid S1(15).All of the fifteen compounds were obtained from the fermented mycelia of G.tsugae for the first time.These results enrich the knowledge of the secondary metabolites of Ganoderma fungi,and provide basis for subsequent pharmacological activity studies.