基于重力驱动液滴定向运输的主动式抗细菌黏附及抗菌研究

Active Anti-bacterial Adhesion and Anti-bacterial Research Based on Gravity Driven Droplet Directional Transportation

有害细菌黏附在各种材料表面会时刻危害着人类生命健康,每年为应对有害细菌黏附所付出的经济成本数以亿计。黏附在材料表面的细菌最终将形成难以清除的生物膜,预防细菌在初始阶段的黏附可以显著减少生物膜的形成,这是应对细菌黏附的有效途径。迄今为止,预防细菌的初始黏附所采用的手段包括超疏水表面、超亲水表面、润滑表面和智能响应型防黏附表面等。但上述提到的绝大多数抗细菌黏附手段属于静态的被动式抗细菌黏附策略,本研究提出了一种基于重力驱动实现液滴定向运输的主动式抗细菌黏附策略,并且结合银纳米粒子负载的杀菌区构建了一种新型主动式抗细菌黏附系统。通过实验探究了微坡高度、倾角与液滴运动的关系,并得到了最优条件。动态抗细菌黏附测试发现对大肠杆菌和金黄色葡萄球菌的抗黏附率高达99.71%和98.79%,具有良好的主动抗细菌黏附效果,验证了重力驱动细菌液滴定向运输主动式抗细菌黏附策略的有效性。末端杀菌区不但具有良好的被动抗黏附效果,并且可以有效地杀死运动至杀菌区的细菌液滴,对大肠杆菌和金黄色葡萄球菌的抗菌率高达100%和100%。本研究提出的基于重力驱动实现液滴定向运输的主动式抗细菌黏附策略的抗黏附效果显著,构建的整个新型主动式抗细菌黏附系统具有良好的抗细菌黏附和杀菌能力,为应对细菌黏附提供了一种全新的策略,在生物医学、微流控技术和工业生产等领域具有重要的应用前景。

Harmful bacteria adhering to various material surfaces can constantly endanger human life and health,and the economic cost of dealing with harmful bacterial adhesion is billions of dollars each year.Bacteria adhering to the surface of materials will eventually form biofilms that are difficult to remove.Preventing bacterial adhesion in the initial stage can significantly reduce biofilm formation,which is an effective way to cope with bacterial adhesion.So far,the methods used to prevent initial bacterial adhesion include superhydrophobic surfaces,superhydrophilic surfaces,lubricating surfaces,and intelligent responsive anti adhesion surfaces.However,the vast majority of anti bacterial adhesion methods mentioned above belong to static passive anti bacterial adhesion strategies.This study proposes an active anti bacterial adhesion strategy based on gravity driven targeted droplet transportation,and constructs a new active anti bacterial adhesion system by combining the sterilization zone loaded with silver nanoparticles.The relationship between micro slope height,inclination angle and droplet movement was explored through experiments,and the optimal conditions were obtained.The dynamic anti bacterial adhesion test found that the anti adhesion rates against E.coli and S.aureus were as high as 99.71%and 98.79%,demonstrating a good active anti bacterial adhesion effect,verifying the effectiveness of the gravity driven bacterial droplet directed transportation active anti bacterial adhesion strategy.The terminal sterilization zone not only has a good passive anti adhesion effect,but also can effectively kill bacterial droplets moving to the sterilization zone,with antibacterial rates of up to 100%and 100%against E.coli and S.aureus.The active anti bacterial adhesion strategy based on gravity driven targeted droplet transportation proposed in this study has significant anti adhesion effects.The entire new active anti bacterial adhesion system constructed has good anti bacterial adhesion and sterilization capabilities,providing a new strategy for dealing with bacterial adhesion.It has important application prospects in biomedical,microfluidic technology,and industrial production fields.