超声提取金银花中绿原酸的正交试验设计

Orthogonal Experimental Design of Ultrasonic Extraction of Chlorogenic Acid in Honeysuckle

目的:为了获得金银花中绿原酸的最佳提取工艺参数。方法:对浸渍法和超声法两种不同的提取方法的效果进行比较,设计了超声法提取金银花中绿原酸的单因素试验和正交试验,对A乙醇浓度(40%、50%、60%)、提取时间(20 min、35 min、50 min)、超声温度(50℃、60℃、80℃)和液料比(20倍、35倍、40倍)这四个因素三个水平,考察其工艺参数的不同对金银花中绿原酸收率的影响和大小。结果:超声法提取金银花中绿原酸的单因素试验结果显示最佳乙醇浓度为50%,最佳时间是35 min,最佳温度是60℃,最佳液料比为35倍;正交试验影响因素大小为A>D>C>B,最佳提取工艺方案为A_(2)B_(2)C_(2)D_(2)。结论:超声法提取金银花所获得的绿原酸得率要比浸渍法金银花所获得的绿原酸得率要高,产率提高了0.19%;超声提取绿原酸的最佳工艺参数是乙醇浓度50%,提取时间35 min,超声提取温度是60℃,液料比是35倍。本工艺参数的优化,为使用超声法单因素试验和正交试验提取贵州金银花中的绿原酸提供了理论依据和数据支持。

Objectiv:In order to obtain the optimum extraction process parameters of chlorogenic acid in honeysuckle.Methods:The effects of two different extraction methods,impregnation method and ultrasonic method,were compared.The single factor test and orthogonal test of ultrasonic extraction of chlorogenic acid in honeysuckle were designed.The concentration of A ethanol(40%,50%,60%),extraction time(20 min,35 min,50 min),ultrasonic temperature(50℃,60℃,80℃)and liquid-solid ratio(20 times,35 times,40 times)were investigated.The effects of different process parameters on the yield of chlorogenic acid in honeysuckle were investigated.Results:The single factor test results of ultrasonic extraction of chlorogenic acid in honeysuckle showed that the best ethanol concentration was 50%,the best time was 35 min,the best temperature was 60℃,and the best liquid-solid ratio was 35 times;the influencing factors of orthogonal test were A>D>C>B,and the best extraction process was A_(2)B_(2)C_(2)D_(2).Conclusion:The yield of chlorogenic acid obtained by ultrasonic extraction of honeysuckle is higher than that obtained by impregnation of honeysuckle,and the yield is increased by 0.19%.The optimum technological parameters of ultrasonic extraction of chlorogenic acid were as follows:ethanol concentration 50%,extraction time 35 min,ultrasonic extraction temperature 60℃,liquid-solid ratio 35 times.The optimization of the process parameters provides a theoretical basis and data support for the extraction of chlorogenic acid in Guizhou honeysuckle by ultrasonic single factor test and orthogonal test.