基于网络药理学和分子对接探究灵芝增强鸡免疫功能的作用机制

Exploring mechanism of Ganoderma lucidum enhancing chicken immune function based on network pharmacology and molecular docking

为探究灵芝增强鸡免疫功能的作用机制,通过TCMSP等数据库获得鸡免疫功能相关基因与灵芝主要有效成分靶点,并利用Cytoscape 3.10.0软件可视化分析灵芝-靶点-免疫蛋白质互作网络(PPI),进而通过David数据库进行GO和KEGG通路富集分析,利用AutoDock软件进行分子对接验证关键有效成分与关键靶点的结合活性。结果显示:共获得灵芝有效成分42个,包括环氧灵芝醇B、赤芝孢子内酯B、灵芝醛B等10个关键有效成分,免疫相关基因332个。PPI包括白细胞介素-6 (IL-6)、白细胞介素-1β (IL-1β)、非受体酪氨酸激酶(SRC)等7个关键靶点。GO功能富集分析涉及生物过程23个、细胞组分22个以及分子功能23个;KEGG通路富集分析获得11条相关信号通路。分子对接的最小结合能均小于-20.9 kJ/mol,表明对接结果良好。研究表明,灵芝增强鸡免疫功能的关键有效成分为10个,关键靶点为7个,相关信号通路为11条,研究结果可为灵芝作为饲料添加剂的应用提供参考。

To investigate the mechanism by which Ganoderma lucidum enhances the immune function in chickens,genes related to chicken immune function and the main effective components of Ganoderma lucidum targets were obtained through databases such as TCMSP.Visualization analysis of the Ganoderma lucidum-target-immune protein-protein interaction(PPI)network was performed using Cytoscape 3.10.0 software,followed by GO and KEGG pathway enrichment analysis through the David database.Finally,molecular docking was conducted using AutoDock software to verify the binding activity of key effective components with key targets.The results showed that a total of 42 effective components of Ganoderma were identified,including 10 key effective components such as Ganoderma lucidum alcohol B,Ganoderma spore lactone B,and Ganoderma lucidum aldehyde B,along with 332 immune-related genes.The PPI network included seven key targets such as Interleukin-6(IL-6),Interleukin-1 beta(IL-1β),and non-receptor tyrosine kinase(SRC).The GO functional enrichment analysis covered 23 biological processes,22 cellular components,and 23 molecular functions.The KEGG pathway enrichment analysis yielded 11 related signaling pathways.The minimum binding energy of the molecular docking was all less than-20.9 kJ/mol,indicating good docking results.The study suggests that the key effective components of Ganoderma that enhance chicken immune function are 10,the key targets are 7,and the related signaling pathways are 11,the results can provide a reference for the application of Ganoderma as a feed additive.