载纳米氧化锌定向纤维膜促进肌腱细胞增殖分化的研究

Study on The Promotion of Tenocyte Proliferation and Differentiation by Oriented Fiber Membrane Loaded With Nano-zinc Oxide

目的为模拟肌腱组织的显微结构和力学性能,促进肌腱组织的再生修复,制备负载不同质量分数纳米氧化锌且同时具备取向结构的左旋聚乳酸(PLLA)纤维膜,对其进行理化表征和生物性能评价,探讨其对肌腱细胞增殖分化的影响。方法利用静电纺丝技术制备PLLA纤维支架及含不同质量分数纳米ZnO的PLLA/ZnO纤维支架。通过扫描电镜、力学拉伸、能谱仪(EDS)图谱表征支架的理化性能,并将支架与小鼠肌腱细胞共培养检测其生物相容性及对细胞增殖、分化的调控作用。结果纤维支架均呈取向性排列,锌元素在纤维中均匀分布,PLLA/0.1%ZnO纤维支架拉伸强度和杨氏模量均显著高于PLLA组。PLLA/0.1%ZnO纤维支架表面细胞数量显著高于PLLA组,且活性更好;小鼠肌腱细胞沿纤维排列方向呈定向性黏附和生长。结论取向PLLA/0.1%ZnO纤维支架具有优良的理化性能,并可显著促进肌腱细胞定向生长和增殖分化,未来有望用于肌腱组织的再生修复。

Objective To simulate the microstructure and mechanical properties of tendon tissue and promote its regeneration and repair,electrospinning technology was used to prepare L-polylactic acid(PLLA)fiber membranes loaded with different nano zinc oxide contents and with oriented structures.Physical and chemical characterization and biological performance evaluation were carried out to explore their effects on tendon cell proliferation and differentiation.Methods Preparation of PLLA fiber scaffolds and PLLA/ZnO fiber scaffolds containing different mass fractions of nano ZnO was performed using electrospinning technology.The physicochemical properties of the scaffold were characterized by scanning electron microscopy,mechanical stretching,and EDS spectroscopy.The scaffold was co-cultured with mouse tendon cells to detect its biocompatibility and regulatory effects on cell differentiation behavior.Results The fiber scaffolds were arranged in an oriented manner,and zinc elements were uniformly distributed in the fibers.The tensile strength and Young’s modulus of PLLA/0.1%ZnO fiber scaffolds were significantly higher than PLLA fiber scaffolds.The number of cells on the surface of PLLA/0.1%ZnO fiber scaffold was significantly higher than that of the PLLA group,and the activity was better;mouse tendon cells exhibit directional adhesion and growth along the fiber arrangement direction.Conclusion The oriented PLLA/0.1%ZnO fiber scaffold had excellent physicochemical properties,which can significantly promote the oriented growth,proliferation and differentiation of tendon cells.It is expected to be used for tendon tissue regeneration and repair in the future.