基于转录组与染色质开放性分析青稞对白粉病胁迫的响应

Based on transcriptional and chromatin accessibility analysis revealing hulless barley response to powdery mildew stress

【目的】探究白粉病胁迫对青稞基因表达和染色质开放性的影响及青稞白粉病胁迫响应分子机制。【方法】对青稞白粉病高抗品种GND7和高感品种ZQ13进行白粉菌侵染和空白处理(利用水替代侵染),收集青稞叶片进行转录组测序和染色质开放性测序,筛选差异表达基因与差异开放性区域。【结果】ZQ13样本白粉菌侵染前后得到1647个差异基因,其中980个基因在侵染过程中上调表达,667个基因在侵染过程中下调表达。针对白粉菌侵染过程中染色质开放性研究发现,ZQ13样本侵染前后共获得7310个差异开放性区域,其中424个区域开放性上调,6886个区域开放性下调。GND7样本白粉菌侵染前后得到1119个差异基因,其中657个基因在侵染过程中上调表达,462个基因在侵染过程中下调表达。针对白粉菌侵染过程中染色质开放性研究发现,GND7侵染前后共获得2573个差异开放性区域,其中1343个区域开放性上调,1230个区域开放性上调。通过转录组和染色质开放性联合分析,筛选出AP2/ERF转录因子(HOVUSG2735700)、MYB1R1转录因子(HOVUSG4226000)等。【结论】青稞在白粉菌侵染过程中基因表达量与染色质开放性发生了较大变化,AP2/ERF转录因子(HOVUSG2735700)、MYB1R1转录因子(HOVUSG4226000)等可能在青稞响应白粉病胁迫中发挥重要作用。

【Objective】The study aimed to investigate the effects of powdery mildew stress on gene expression and chromatin accessibility in hulless barley,and to elucidate the molecular mechanisms underlying hulless barley's response to this stress.【Method】Two hulless barley varieties,GND7(powdery mildew-resistant)and ZQ13(powdery mildew-sensitive),were exposed to powdery mildew infection and a control treatment(using water instead of infection).Leaf samples were then collected for transcriptome and chromatin accessibility sequencing to identify differentially expressed genes and differentially accessible regions.【Result】In ZQ13,1647 differentially expressed genes were found,comprising 980 upregulated and 667 downregulated genes during infection.Chromatin accessibility analysis revealed 7310 differentially accessible regions,with 424 regions showing increased accessibility and 6886 regions showing decreased accessibility after infection.In GND7,1119 differentially expressed genes were identified,with 657 upregulated expression and 462 downregulated expression.Chromatin accessibility analysis indicated 2573 differentially accessible regions,with 1343 regions showing increased and 1230 regions showing decreased accessibility post-infection.Key genes,such as the AP2/ERF transcription factor(HOVUSG2735700)and MYB1R1 transcription factor(HOVUSG4226000),were identified through integrated transcriptome and chromatin accessibility analyses.【Conclusion】Hulless barley exhibits significant alterations in gene expression and chromatin accessibility in response to powdery mildew infection.AP2/ERF transcription factor(HOVUSG2735700)and MYB1R1 transcription factor(HOVUSG4226000)may play crucial roles in the plant's response to powdery mildew stress.