农业科技支撑巩固拓展脱贫攻坚成果同乡村振兴有效衔接研究——以四川民族地区为例

Effects of Mito-TEMPO on cryopreservation of porcine spermatozoa

巩固拓展脱贫攻坚成果同乡村振兴有效衔接是新时期“三农”工作最重要的任务之一,而农业科技对乡村产业振兴起着关键作用。四川民族地区普遍存在农业科技创新投入强度不够、现代农业基础薄弱、农业科技人才供给不足、农业科技创新机制不完善、农户科技文化素质相对偏低、内生动力不足等问题,建议从构建农业科技创新和成果转化同时发力的科技支撑体系,建设高能级科技成果示范转化平台提升科技供给水平,完善农业生产经营组织体系,完善农业信息化和机械化服务体系,建立农产品质量安全与标准化体系,强化农业科技人才培养体系六个方面入手,实现四川省少数民族地区巩固拓展脱贫攻坚成果同乡村全面振兴有力有效精准衔接。

【Objective】The study aimed to select the optimal concentration of Mito-TEMPO.Meanwhile,the effect of Mito-TEMPO on the quality,antioxidation capacity,and mitochondrial function of frozen-thawed porcine spermatozoa were explored to provide a reference basis for the improvement of porcine spermatozoa cryopreservation.【Method】0.5,5.0,50.0 and 500.0μmol/L Mito-TEMPO were added to the frozen solutionⅠin the experimental group.The concentration of the Mito-TEMPO suitable for the cryopreservation of porcine spermatozoa was screened by evaluating the kinetic parameters and viability of spermatozoa in each group.The plasma membrane intactness,acrosome intactness,DNA fragmentation index(DFI),reactive oxygen species(ROS)level,total antioxidant capacity(T-AOC),malondialdehyde(MDA)content,catalase(CAT)and total superoxide dismutase(T-SOD)activities,as well as ATP and mitochondrial respiratory chain complexⅠ-V content of freezing-thawing porcine spermatozoa were detected.【Result】Compared to the fresh group(F group),the kinetic parameters and viability of spermatozoa were significantly reduced after freezing-thawing(P<0.05,the same as below).While the addition of Mito-TEMPO improved the kinetic parameters and viability of frozen-thawed spermatozoa to various degrees,and the effect of 50.0μmol/L Mito-TEMPO was the optimal.Compared to the F group,the plasma membrane and acrosome integrity,T-AOC,ATP and mitochondrial respiratory chain complex I-V contents of frozen-thawed spermatozoa were significantly reduced,as well as CAT and T-SOD activities.DFI,ROS levels and MDA contents were significantly increased.Compared to the frozen control group(C group),the plasma membrane integrity,acrosome integrity,T-AOC,as well as ATP and mitochondrial respiratory chain complex I,ⅡandⅤcontents of spermatozoa were significantly increased in the 50.0μmol/L Mito-TEMPO group(M group).Moreover,the DFI,ROS levels and MDA contents were significantly reduced,and the CAT and T-SOD activities were significantly enhanced by the 50.0μmol/L Mito-TEMPO.【Conclusion】Mito-TEMPO has a cryoprotective effect on porcine spermatozoa,and the optimal concentration is 50.0μmol/L.In addition,50.0μmol/L Mito-TEMPO increases the quality of spermatozoa by improving the antioxidation capacity and mitochondrial function of porcine spermatozoa after freezing-thawing.