北桑寄生总黄酮对硫代乙酰胺诱导斑马鱼急性肝损伤的保护作用

Protective effects of total flavonoids from Loranthus tanakae on thioacetamide-induced acute liver injury in zebrafish

目的探讨北桑寄生总黄酮(total flavonoids of Loranthus tanakae,LTF)对硫代乙酰胺(thioacetamide,TAA)诱导的斑马鱼急性肝损伤(acute liver injury,ALI)的保护作用。方法采用TAA诱导AB品系与转基因品系斑马鱼建立ALI模型,通过对斑马鱼的生长发育、肝脏形态、肝功能酶活性、脂质积累情况及氧化应激指标进行检测,考察LTF是否具有保肝活性。结果25、50μg/mL LTF可以明显抑制TAA引起的斑马鱼发育毒性,促进幼鱼生长及减缓卵黄囊的吸收滞后现象(P<0.05、0.01)。与模型组比较,LTF可以明显增加斑马鱼幼鱼肝脏面积,提高肝脏的荧光强度(P<0.05、0.01)。苏木素-伊红染色结果显示,LTF处理后斑马鱼肝脏病理状态得到缓解,空泡化现象明显减少。LTF显著降低TAA诱导的斑马鱼丙氨酸氨基转移酶(alanine aminotransferase,ALT)、天冬氨酸氨基转移酶(aspartate aminotransferase,AST)活性(P<0.05、0.01)。油红O染色结果显示,LTF显著降低幼鱼肝脏区域脂质积累程度(P<0.05、0.01)。氧化应激指标测定结果显示,LTF处理后幼鱼体内的活性氧(reactive oxygen species,ROS)和丙二醛(malondialdehyde,MDA)水平明显降低(P<0.05、0.01),超氧化物歧化酶(superoxide dismutase,SOD)、过氧化物酶(catalase,CAT)和还原型谷胱甘肽(reduced glutathione,GSH)含量显著升高(P<0.05、0.01)。结论LTF在防治ALI方面具有较大的潜力,其肝脏保护活性可能与抗氧化作用有关。

Objective To investigate the protective effect of total flavonoids from Loranthus tanakae(LTF)on thioacetamide(TAA)-induced acute liver injury(ALI)in zebrafish.Methods TAA was used to induce ALI model in zebrafish of AB and transgenic strains.Whether LTF had hepatoprotective activity was assessed by examining the growth and development,liver morphology,liver function enzyme activities,lipid accumulation and oxidative stress indexes of zebrafish.Results 25,50μg/mL LTF significantly inhibited TAA-induced developmental toxicity in zebrafish,promoted growth and slowed down the absorption lag of the yolk sac(P<0.05,0.01).Compared with model group,LTF could significantly increase the liver area and improve the fluorescence intensity of the liver in zebrafish larvae(P<0.05,0.01).The results of hematoxylin eosin staining showed that the liver pathology of zebrafish was alleviated and vacuolization was significantly reduced after LTF treatment.LTF significantly reduced the activities of alanine aminotransferase(ALT)and aspartate aminotransferase(AST)induced by TAA in zebrafish(P<0.05,0.01).The oil red O staining results showed that LTF significantly reduced the degree of lipid accumulation in liver region of larvae(P<0.05,0.01).The measurement results of oxidative stress indicators showed that after LTF treatment,the levels of reactive oxygen species(ROS)and malondialdehyde(MDA)in zebrafish larvae were significantly reduced(P<0.05,0.01),while the levels of superoxide dismutase(SOD),catalase(CAT)and reduced glutathione(GSH)were significantly increased(P<0.05,0.01).Conclusion LTF has great potential in the prevention and treatment of ALI,and its liver protective activity may be related to antioxidant effects.