艾GRF基因家族的鉴定与表达分析

Identification and Expressions of GRF Genes in Artemisia argyi

【目的】生长调节因子(growth-regulating factors,GRF)是植物的一种特有蛋白,探明艾(Artemisia argyi)GRF基因家族的生物学特性,为艾的生长发育和逆境胁迫调节提供依据。【方法】对艾GRF基因家族进行理化性质、进化、基因结构以及表达等生物信息学进行分析,进而通过转录组数据和实时荧光定量PCR(qRT-PCR)技术分析基因家族各成员在不同组织、逆境胁迫以及响应不同激素的表达模式。【结果】从艾基因组中共鉴定出17个GRF家族成员,理化鉴别显示其编码蛋白均为亲水蛋白;系统发育进化树分为4个亚家族;motif分析表明进化树中同一分支的基因具有相似或相同的保守基序;AaGRF基因家族成员随机分布在11条不同的染色体上;AaGRF基因家族启动子含有多种逆境和激素响应元件;Aa GRF基因家族在响应盐碱胁迫时表现出不同的表达趋势;该家族成员在艾的根、茎、叶中的表达模式具有组织特异性;经脱落酸、吲哚乙酸、水杨酸和茉莉酸甲酯处理后,AaGRF05、AaGRF06、AaGRF11和AaGRF14在处理后的12 h基因表达量最高,表明大部分基因在前期参与不同激素的调控。【结论】艾GRF基因家族参与艾生长发育、激素与逆境胁迫调节,为探究GRF家族功能提供的理论依据。

【Objective】Growth-regulating factor(GRF) genes in Artemisia argyi were identified and analyzed.【Method】Bioinformatic analysis on the physiochemical properties,structure,and evolution of the GRF genes,as well as their expressions in different tissues under stresses,of A.argyi was conducted using qRT-PCR.【Result】Seventeen AsGRFs were hydrophilic proteins in the phylogenetic evolutionary tree of 4 subfamilies.Those genes of same clade in the evolutionary tree contained a similar or same conserved motif.The members were randomly distributed on 11 chromosomes with promoters of multiple stress-and hormone-responsive elements and one that varied in the response to salinity.The tissue-specific expressions of the genes,including AaGRF05,AaGRF06,AaGRF11,and AaGRF14,peaked in 12h after being exposed to abscisic acid,indoacetic acid,salicylic acid,or methyl jasmonate.【Conclusion】The GRF genes associated with the growth and stress regulation of A.argyi were identified,and relevant bioinformatics obtained.