基于SLAF-Seq技术的橄榄种质资源SNP标记开发与遗传关系鉴定

SNP Markers Development and Genetic Relationship Identification of Germplasm Resources of Canarium album Based on SLAF-Seq Technology

【目的】开发橄榄SNP标记并分析其种质资源遗传多样性,为橄榄种质资源保护和利用提供依据。【方法】基于SLAF-Seq技术进行橄榄SNP标记开发,同时采用系统进化分析、群体聚类分析和主成分分析等研究了橄榄种质资源遗传结构和遗传多样性。【结果】基于SLAF-Seq技术共挖掘到506 701个SLAF标签,其中多态性SLAF标签27 108个,开发获得361 386个群体SNP标记;基于SNP标记,利用系统进化树和群体聚类分析可分别将橄榄种质资源分为3和6个类群,整体Nei多样性指数和Shanon-Wiener指数分别为0.321和0.472。两种分类方法分析结果均发现,不同地区之间的橄榄种质资源并未严格按照地域分布归类。【结论】橄榄种质资源遗传多样性相对丰富,且不同地域间存在种质资源交流,而采用SNP标记可有效鉴定橄榄种质资源。

【Objective】SNP markers development and genetic diversity analysis of Canarium album were perfomed to facilitate the protection and utilization of germplasm resources of C.album.【Method】SNP markers of C.album were developed based on SLAF-Seq technology,and the genetic structure and genetic diversity were studied by phylogenetic analysis,population cluster analysis and principal component analysis.【Result】A total of 506 701 SLAF loci were mined,including 27 108 polymorphic SLAF loci,and 361 386 SNP markers were developed.Based on these SNP markers,germplasm resources of C.album were respectively divided into 3 and 6 groups by phylogenetic tree and population cluster analysis.The overall Nei diversity index and Shanon Wiener index were 0.321 and 0.472,respectively.The results of two classification methods showed that germplasm resources of C.album from different regions were not classified strictly according to regional distribution.【Conclusion】The genetic diversity of germplasm resources of C.album had great genetic diversity which were exchange frequently among different regions,and the developed SNP markers of C.album could effectively identify the germplasm resources.