EM菌发酵兔粪浸出液对小球藻生长及生化成分的影响

Effects of rabbit feces extract fermented by EM bacteria on the growth and biochemical components of Chlorella vulgaris

研究了EM厨余堆肥菌发酵处理的兔粪浸出液对小球藻(Chlorella vulgaris)的生长及蛋白质、可溶性糖、叶绿素等生化成分的影响。结果表明,兔粪浸出液处理组中,7%浓度组小球藻在第12天时达到最大现存量,为1.06×10^(7)个/mL,而对照组最大现存量只有6.45×10^(6)个/mL;7%浓度组的小球藻与对照组相比生长差异表现明显。5%、7%浓度组在第8天时其藻细胞密度已接近对照组的最大现存量,分别为6.02×10^(6)个/mL和6.80×10^(6)个/mL,表明适宜浓度的兔粪浸出液处理对小球藻的生长有刺激作用。低浓度组中3%浓度组的小球藻生长不理想,但蛋白质积累最高,含量达到17.31 mg/L,与对照组相比差异显著(P<0.05)。6%浓度组的可溶性糖含量最高,为332.7 mg/L,其叶绿素a、叶绿素b和类胡萝卜素含量分别为3.19、1.58 mg/L和0.57 mg/L,与对照组相比差异显著。

The effects of rabbit feces extract fermented by EM kitchen waste composting bacteria on the growth and biochemical components such as protein,soluble sugar and chlorophyll of Chlorella vulgaris were studied.The results showed that in the treatment group of rabbit feces extract,the Chlorella vulgaris in the 7%concentration group reached the maximum standing stock at the 12th day,which was 1.06×10^(7)cells/mL,while the maximum standing stock in the control group was only 6.45×10^(6)cells/mL.The growth performance of Chlorella vulgaris in the 7%concentration group was significantly different from that in the control group.On the 8th day,the algal cell density of 5%and 7%concentration groups were close to the maximum standing stock of the control group,which were 6.02×10^(6)cells/mL and 6.80×10^(6)cells/mL,respectively,indicating that the appropriate concentration of rabbit manure extract treatment could stimulate the growth of Chlorella vulgaris.However,in the low concentration group,the growth of Chlorella vulgaris in the 3%concentration group was not ideal,but the protein accumulation was the highest,reaching 17.31 mg/L,which was significantly different from the control group(P<0.05).The content of soluble sugar in the 6%concentration group was the highest,which was 332.7 mg/L,and the contents of chlorophyll a,chlorophyll b and carotenoid were 3.19 mg/L,1.58 mg/L and 0.57 mg/L,respectively,which were significantly different from those in the control group.