造影剂肾病模型中GSH-px、SOD、MDA及病理组织变化研究

GSH-px,SOD,MDA and pathological tissue changes in contrast nephropathy model

目的研究造影剂肾病(CIN)大鼠模型中血谷胱甘肽过氧化物酶(GSH-px)、超氧化物歧化酶(SOD)、丙二醛(MDA)及病理组织变化,明确氧化应激机制在CIN中的作用。方法选取成年雄性SD大鼠40只,分为3个大组、5个小组,造模结束后,每组取6只状态较好的大鼠进行实验。测定大鼠血清SOD、GSH-px、MDA水平;进行肾脏组织活检,比较肾脏细胞形态学变化。结果空白对照组、对照组、实验组基线资料比较,差异均无统计学意义(P>0.05)。空白对照组、对照组造模前、造模后24h、造模后48h组内血清GSH-px、SOD及MDA水平比较,差异无统计学意义(P>0.05)。实验组造模前与造模后24、48h血清GSH-px、SOD及MDA水平比较,差异有统计学意义(P<0.05);实验组造模后24h和造模后48h血清GSH-px、SOD及MDA水平比较,差异无统计学意义(P>0.05)。3组在造模后24h的血清GSH-px、SOD及MDA比较,差异无统计学意义(P>0.05)。造模后48h3组及各组两两比较血清GSH-px、SOD及MDA水平,差异有统计学意义(P<0.05)。空白对照组及对照组病理切片显示,肾小球、肾小管及肾间质等均未见明显异常变化。实验组造模后24h可见肾间质纤维化及炎症细胞浸润,但肾小管未见明显改变;48h后,可见明显肾小管中度灶状萎缩,上皮细胞颗粒变性及空泡样改变。结论氧化应激机制在CIN中存在一定作用,造影剂主要作用于肾小管及肾间质,对于肾小球无明显损伤。

Objective To study the changes of blood glutathione peroxidase(GSH-px),superoxide dismutase(SOD),malondialdehyde(MDA)and pathological tissues in the rat contrast-induced nephropathy(CIN)model,and to determine the role of oxidation mechanism in CIN.Methods A total of 40 adult male SD rats were selected and divided into three big groups and five small groups.After constructing the model,six rats with good status were taken from each group for conducting the experiment.The serum GSH-px,SOD and MDA levels were measured,the renal tissue biopsy was performed and the morphological changes of kid-ney cells were compared.Results There was no statistically significant difference in the baseline data among the blank control group,the control group and the experimental group(P>0.05).There was no statistically significant difference in serum GSH-px,SOD and MDA levels before model construction,at 24,48 h after model construction between the blank control group and the control group(P>0.05).There were statistically significant differences in serum GSH-px,SOD and MDA levels of the experimental group between before model construction and after model construction(P<0.05).There was no statistically significant difference in serum GSH-px,SOD and MDA level in the experimental group between at 24 h after modelling and 48 h after modeling(P>0.05).There was no statistically significant difference in serum GSH-px,SOD and MDA levels at 24 h after modeling among the three groups(P>0.05).There were statistically significant differences in serum GSH-px,SOD and MDA levels at 48 h after modeling among the three groups and their pairs(P<0.05).The pathological sections of the blank control group and control group showed no obvious abnormal changes in glomeruli,renal tubule and renal interstitium.Renal interstitial fibrosis and inflammatory cell infiltration were seen after 24 h in the experimental group,but there was no obvious change in the renal tubules.After 48 h,moderate focal-like atrophy of renal tubules,epithelial cell granule degeneration and vacuolar changes were obviously seen.Conclusion The oxidative stress mechanism plays a role in CIN.The contrast agent acute renal injury mainly acts on the renal tubules and renal interstitium,and there is no obvious damage to the glomeruli.