超声波辅助低共熔溶剂提取甜樱桃色素及其功能特性分析

Ultrasonic-assisted deep eutectic solvents extraction of sweet cherry pigments and its functional characteristics analysis

目的 优化超声波辅助低共熔溶剂(deep eutectic solvents,DESs)提取甜樱桃色素的工艺条件,并研究甜樱桃色素对α-淀粉酶和蔗糖酶的抑制特性以及体内抗氧化能力的影响。方法 筛选最佳DESs,考察DESs含水量、固液比、超声功率和提取时间对甜樱桃色素提取效果的影响,测定甜樱桃色素对α-淀粉酶、蔗糖酶的抑制率及对秀丽隐杆线虫衰老的影响。结果 氯化胆碱与1,4-丁二醇的摩尔比为1:3时制备的DESs为最佳溶剂,超声波辅助DESs提取甜樱桃色素的最佳工艺参数为:DESs含水量20%、固液比1:40 (g/m L)、超声功率316 W、提取时间为41 min,该工艺下甜樱桃色素的平均提取量为(465.10±1.46) mg C3G/100 g,显著优于70%乙醇的提取量。甜樱桃色素在100μg/m L的条件下对α-淀粉酶和蔗糖酶的抑制率分别为80.20%±0.65%和73.50%±1.26%。甜樱桃色素能显著延长秀丽隐杆线虫在过氧化氢胁迫下的存活率(P<0.05),平均寿命达(8.41±0.31) h,显著高于阴性对照组[(2.67±0.20) h]和阳性对照组[(5.23±0.17) h]。甜樱桃色素还显著提高了总超氧化物歧化酶(>60.71%)和过氧化氢酶(>43.10%)的活性,降低了丙二醛水平(P<0.05)。结论 超声波辅助DESs提取甜樱桃色素方法 可行,甜樱桃色素可抑制葡萄糖苷酶活性,能够减轻体内氧化应激损伤,具有很大的开发利用价值。

Objective To optimize ultrasonic-assisted deep eutectic solvents(DESs)extraction process for preparing sweet cherry pigment,and investigate the inhibitory properties of sweet cherry pigments onα-amylase and sucrase,as well as their impact on antioxidant capacity in vivo.Methods Select the best DESs,investigate the effects of DESs water content,solid-liquid ratio,ultrasonic power and extraction time on the extraction effect of sweet cherry pigment,and determine the inhibition rate of sweet cherry pigment onα-amylase and sucrase,as well as the effects of aging of Caenorhabditis elegans.Results The results showed that,the DESs prepared at a molar ratio of choline chloride to 1,4-butanediol of 1∶3 was the best solvent,the optimum technological parameters of ultrasonic-assisted DESs extraction of sweet cherry pigment were as follows:The water content of DESs was 20%,solid-liquid ratio 1∶40(g/mL),ultrasound power was 316 W,extraction time was 41 min,and the average amount of pigment extracted from the optimized extraction was(465.10±1.46)mg C3G/100 g,significantly better than 70%ethanol extraction yield.The inhibition rate of the pigments toα-amylase and sucrase was 80.20%±0.65%and 73.50%±1.26%,respectively,at the concentration of 100μg/mL sweet cherry pigment.Sweet cherry pigment could significantly prolong the survival rate of Caenorhabditis elegans under hydrogen peroxide stress(P<0.05),and the mean lifespans of Caenorhabditis elegans reached(8.41±0.31)h,which was significantly higher than that of the negative control[(2.67±0.20)h]and the positive control group[(5.23±0.17)h].Sweet cherry pigment also significantly enhanced the activities of total superoxide dismutase(>60.71%)and catalase(>43.10%)and reduced the level of malondialdehyde(P<0.05).Conclusion The results indicate that ultrasound-assisted DESs extraction of sweet cherry pigment is feasible,the sweet cherry pigment can inhibit the activity of glucosidase and reduce oxidative stress damage in vivo,which has great development and utilization value.