花胶中17种氨基酸及蛋白质系数的测定

Determination of 17 Amino Acids and Protein Coefficients in Fish Maw

对花胶中17种氨基酸及蛋白质系数进行测定研究,建立氨基酸分析仪的方法对花胶中的17种氨基酸进行测定。样品用盐酸加热水解后,使用磺酸型钠离子分离柱进行分离,使用茚三酮对17种氨基酸进行柱后在线衍生。17种氨基酸均使用单点法进行定量分析,添加水平为15, 30和75 mg/kg的平均回收率结果为80.2%~88.2%,相对标准偏差S_(RSD) (n=6)为6.78%~8.67%,方法检出限为1.0~4.9 mg/kg,定量限为3.2~14.9 mg/kg。通过测定每种氨基酸的含量比例,结合其氮元素的质量比,计算得出花胶中的蛋白质系数为6.02,与理论值相近。该方法操作快速高效、回收率高、重现性好,可用于不同食品中氨基酸及蛋白质系数的测定。

A study was conducted on the determination of 17 amino acids and protein coefficients in fish maw.A amino acid analyzer method was established for determination of 17 amino acids in fish maw.After heating and hydrolysis with hydrochloric acid,the samples were separated by sulfonic acid type sodium ion column,and 17 amino acids were subjected to post column online derivatization with ninhydrin.About 17 amino acids were quantitatively analyzed using the single point method with the average recovery rate ranged from 80.2%to 88.2%,the spiked level was 15,30 and 75 mg/kg,while the relative standard deviations(S_(RSD),n-6)of6.78%to 8.67%.The limit of detection(LOD)for 17 amino acids was 1.0 mg/kg to 4.9 mg/kg,and the limit of quantitation(LOQ)was 3.2 mg/kg to 14.9 mg/kg.According to results of the proportion of each amino acid content and combined with the mass ratio of nitrogen elements,the protein coefficient in the fish maw was calculated to be 6.02,which was close to the theoretical value.This method was fast and efficient to conduct with high recovery rate and good reproducibility,and could be used for the determination of amino acid and protein coefficients in different foods.