响应面优化鹅肠菜多酚超声波辅助提取工艺及其对亚硝酸盐的清除作用

Optimization of ultrasonic-assisted extraction technology by response surface methodology and antioxidant activity of polyphenols from Myosoton aquaticum

为提高鹅肠菜资源的附加值,对鹅肠菜多酚进行了超声辅助提取并考察了多酚的活性.首先考察了超声温度、液料比、乙醇体积分数和超声时间4个单因素对鹅肠菜多酚提取率的影响,进一步采用了Box-Behnken实验设计对提取工艺进行响应面优化,最后考察了鹅肠菜多酚对亚硝盐的清除作用.结果表明鹅肠菜多酚的最佳提取工艺参数为:超声温度73℃,液料比33 mL/g,乙醇体积分数63%,超声时间41 min.在这种条件下,鹅肠菜多酚提取率的平均值为88.97 mg/g,与模型预测值(89.58 mg/g)相比,相对误差仅为0.68%,表明该模型是有效且可靠.鹅肠菜多酚具有一定的亚硝酸盐清除能力,且与亚硝酸盐的清除率之间存在一种量效关系,其半抑制浓度(IC 50)为1.82 mg/L.该研究为鹅肠菜的综合开发利用提供了新的途径.

The aim was to improve the added value of Myosoton aquaticum resources,ultrasonic-assisted extraction of polyphenols from Myosoton aquaticum was carried out and the activity of polyphenols was investigated.Firstly,the effects of ultrasonic temperature,liquid-solid ratio,ethanol concentration and ultrasonic time on the extraction yield of polyphenols from Myosoton aquaticum were investigated,and then the extraction process was optimized by Box-Behnken experimental design.Finally,the scavenging effect of polyphenols from Myosoton aquaticum on nitrite was investigated.The results showed that the optimum extraction process parameters were as follows:ultrasonic temperature 73℃,liquid-solid ratio 33 mL/g,ethanol concentration 63 and ultrasonic time 41 min.Under these conditions,the average extraction rate of polyphenols from Myosoton aquaticum was 88.97 mg/g.Compared with the predicted value of the model(89.58 mg/g),the relative error was only 0.68%,which showed that the model was effective and reliable.Polyphenols from Myosoton aquaticum had a certain nitrite scavenging ability,and its IC 50 value was 1.82 mg/L,and there was a dose-effect relationship with nitrite scavenging rate.This study provided a new way for the comprehensive development and utilization of Myosoton aquaticum.