西番莲种间杂交F1后代分子鉴定及遗传多样性分析

Passiflora caerulea L.Interspecific Hybrid F1 Progeny Molecular Identification and Genetic Diversity Analysis

以红花西番莲为母本、大果西番莲为父本进行种间杂交,对杂交F1代群体植物学性状进行观察和调查,利用筛选出的5对扩增结果稳定且具多态性的SRAP引物,对杂交F1代群体进行杂种鉴定,并对亲本和杂交F1代群体的基因分离规律以及遗传多样性和遗传关系进行分析。结果显示:杂交F1代29个单株的藤蔓形状、蜜腺位置以及叶片形状等均发生变化,表现出不同程度的多样性;SRAP分子鉴定结果表明,5对SRAP引物可以将29个杂交单株鉴定出来,说明这些单株为真杂种。采用UPGMA法构建系统聚类图,结果表明,在遗传相似系数0.60处可将父母本及29株杂交F1代植株分为3类,其中第1类与母本“hh”聚在一起,共有杂交F1代植株12株;第2类与父本“dg”聚在一起,共有杂交F1代植株11株;第3类有杂交F1代植株6株。综合分析结果表明:采用SRAP分子标记法可对西番莲的杂交F1代群体进行有效的杂种鉴定。

Interspecific hybridization was carried out with the red flower Passiflora as the mother and the large fruit Passiflora as the father.Through the observation and investigation of the botanical characteristics of the hybrid F1 generation population,five pairs of SRAP primers with stable amplification results and polymorphisms were selected to identify the F1 hybrid generation population.The rule of gene separation,genetic diversity,and genetic relationships between the parents and the hybrid F1 generation population were analyzed.The results showed that the vine shape,nectary position,and leaf shape of 29 single plants in the F1 generation were separated,indicating different degrees of diversity.The results of SRAP molecular identification showed that five pairs of SRAP primers could be used to identify 29 single hybrid strains,illustrating that these single strains were true hybrids.The UPGMA method was used to construct the system cluster graph.The results showed that at a genetic similarity coefficient of 0.60,the parents and 29 hybrid F1 generation plants could be divided into three types,among which the first type was grouped with the mother“hh”and a total of 12 hybrid F1 generation plants,the second type was grouped with the father“dg”and a total of 11 hybrid F1 generation plants,and the third type had six hybrid F1 generation plants.The results showed that the SRAP could be used to identify the F1 hybrids of Passiflora.