光照强度对黄精幼苗生长和生理的影响

Effects of Light on the Growth and Physiology of Three Strains of Polygonati rhizoma Seedlings

为确定黄精幼苗健壮生长所需要的光照强度,以26号、83号、91号3个品系黄精组培苗为实验材料,以2000 lx为对照,设计4000、6000、8000、10000、20000 lx光照强度,检测其生长和生理指标。研究结果显示,光照对不同品系黄精幼苗生长的影响不同,当光照强度在6000 lx时生长最好,根长相对较短,26号、91号的根数最多;83号地上与地下部分鲜重比先升高后降低。3个品系组培苗折干率受光照影响极显著(p<0.01),其中光照强度大于8000 lx折干率显著增加。随着光照强度的增加,组培苗的超氧化物歧化酶(superoxide dismutase,SOD)、过氧化物酶(peroxidase,POD)活性先升高后降低,可溶性蛋白含量呈升高降低再升高趋势。随着光照强度的增强,91号品系叶绿素呈单峰变化趋势,当光照强度为6000 lx时,叶绿素(a+b)含量最高;26号品系叶绿素含量呈“V”形变化趋势,光照强度为20000 lx时含量最高;83号品系呈倾斜的“N”形变化趋势,也在光照强度达20000 lx时叶绿素含量最高。光照强度达6000 lx时能提高黄精种苗质量,26号品系黄精在强光环境下适应性较强。

To determine the required light intensity for the robust growth of Polygonatum sibiricum tissue-culture-generated plants,three strains of P.sibiricum tissue-culture-generated plants,26,83,and 91,were used as experimental materials.With 2000 lx as the control,4000,6000,8000,10000,20000 lx light intensities were used to measure the growth and physiological indices of the plants.The results showed that the effect of light on the growth of different strains of tissue-cultured plants differed.When the light intensity was 6000 lx,the growth of the tissue-cultured plants was the greatest,with shorter root length pairs and the 26 and 91 strains had the greatest number of roots.The fresh weight ratio of the aboveground and underground parts of strain 83 first increased and then decreased.The drying rate of the three strains of tissue-cultured seedlings was significantly affected by the light(p<0.01),and the drying rate significantly increased when the light intensity was greater than 8000 lx.With increasing light intensity,the superoxide dismutase(SOD)and peroxidase(POD)enzyme activities of tissue-cultured seedlings first increased and then decreased.The soluble protein content tended to increase,decrease,and then increase.With increasing light intensity,the chlorophyll content of strain 91 showed a single peak trend,with the highest chlorophyll(a+b)content occurring at a light intensity of 6000 lx;those of 26 strain show a“V”shaped trend,with the highest content at a light intensity of 20000 lx;and those of 83 strains showed a tilted“N”shaped trend of change,and the content was greatest when the illumination reached 20000 lx.When the illumination reached 6000 lx,the quality of P.sibiricum seedlings improved;26 P.sibiricum strains exhibited strong adaptability in high-light environments.