MATN3的表达对胃癌细胞侵袭迁移能力的影响及EMT的相关性研究

Effect of MATN3 expression on invasion and migration ability of gastric cancer cells and correlation study of EMT

目的:探讨胃癌细胞中人母系蛋白3(MATN3)的表达变化对胃癌细胞侵袭迁移能力及上皮间质转化(EMT)的影响,方法:检测不同胃癌细胞株中的MATN3的表达,并检测MATN3的沉默和过表达效果,及沉默和过表达MATN3后的胃癌细胞侵袭迁移能力的变化、胃癌细胞EMT相关蛋白的表达变化。结果:MATN3在不同胃癌细胞株中的表达存在差异性;沉默MATN3,胃癌细胞的侵袭迁移能力明显降低(P<0.01),过表达MATN3,胃癌细胞的侵袭迁移能明显增强(P<0.01);沉默MATN3,EMT相关蛋白E-Cadherin的表达降低,N-Cadherin的表达升高(P<0.01);过表达MATN3,E-Cadherin的表达升高,N-Cadherin的表达降低(P<0.01)。结论:MATN3的表达变化导致了胃癌细胞的侵袭迁移能力的改变,并导致EMT相关蛋白表达改变,从而影响了胃癌细胞的EMT过程。

Objective:To investigate the effect of martrilin3(MATN3)expression on invasion and migration ability and epithelial mesenchymal transition(EMT)of gastric cancer cells.Methods:The expression of MATN3 in different gastric cancer cell lines was detected.The efficiency of silencing and overexpressing MATN3 was assessed.The changes of invasion and migration ability,and expression of EMT-related proteins of gastric cancer cells after silencing and overexpressing MATN3 were detected.Results:There were differences in the expression of MATN3 in different gastric cancer cell lines.Silencing MATN3 resulted in a decrease in the invasion and migration ability of gastric cancer cells(P<0.01),while overexpression of MATN3 resulted in an increase in the invasion and migration ability of gastric cancer cells(P<0.01).Silencing MATN3 led to decreased expression of EMT-related protein E-Cadherin and increased expression of N-Cadherin,while overexpression of MATN3 led to increased expression of E-Cadherin and decreased expression of N-Cadherin(P<0.01).Conclusions:The change of MATN3 expression leads to the change of invasion and migration ability of gastric cancer cells and the change of EMT-related protein expression,which affects the EMT process of gastric cancer cells.