五味子多糖对家兔皮肤弹性的影响

Experimental study on the effect of Schisandra chinensis polysaccharides on skin elasticity in rabbits

目的:观察五味子多糖(SCP)对家兔皮肤弹性的影响。方法:利用水提醇沉法提取SCP。选取雄性新西兰兔6只。每只家兔腹部剃毛并标记划分为五个区域。五个区域分别对应空白,基质,3%、5%和10%SCP。空白区域不进行任何处理,基质区域涂抹卡波姆溶液,SCP区域按不同浓度进行分区涂抹,以7 d为1个周期,连续给药14 d。在第7、14天涂抹后,间隔12 h进行弹性测试,用皮肤弹性分析仪对家兔涂抹区域进行弹性测定。检测每一区域的弹性值R2、R6、R7的数值,就不同浓度的药物对上述数值的影响进行数据分析。结果:各组弹性值检测结果显示,基质干预组与空白对照组相比,其R2、R7数值均显著增高(P<0.01或P<0.05),其R6数值显著降低(P<0.01或P<0.05)。基质干预组对比SCP给药组,其R2、R7数值均显著下降(P<0.01或P<0.05),其R6数值显著升高(P<0.01或P<0.05)。对比SCP给药组的弹性数值发现,10%SCP相较3%与5%SCP,其R2、R7数值均显著增高(P<0.01或P<0.05),其R6数值显著降低(P<0.01或P<0.05)。结论:SCP药膏对家兔的皮肤有保护作用。

Objective To observe the effect of Schisandra chinensis polysaccharide(SCP)on rabbit skin elasticity to provide theoretical basis for the research and development of related skin care products.Method Schisandra crude polysaccharide was extracted by water alcohol ol.Six male New Zealand rabbits were selected.Each rabbit is shaved and divided into five areas.The five regions correspond to blank,matrix,3%,5%and 10%pentapolysaccharides.No treatment was performed in the blank area,the capom solution was applied in the matrix area,and the pentpolysaccharide area was partitioned at different concentrations,taking 7 days and administered continuously for 14 days.After days 7,14,12 h intervals and the rabbit smear area was tested with a skin elasticity analyzer.Detection of values of elastic value R2,R6,R7 in each region and data analysis on the effects of the drug of different concentrations on the above values.Results The elastic detection results of each group showed a significant R2,R7 in the matrix intervention group compared to blank controls(P<0.01 or P<0.05)and a significant R6(P<0.01 or P<0.05).The R2,R7 values all decreased significantly in the matrix intervention group(P<0.01 or P<0.05)and the R6 value increased significantly(P<0.01 or P<0.05).Compared with the elastic values of the pentaflavor polysaccharide administration group,we found that 10%concentration compared with 3%and 5%concentration had significantly increased R2,R7 value(P<0.01 or P<0.05),significantly reduced R6 value(P<0.01 or P<0.05),showing the recovery ability of high dose concentration of polysaccharide ointment to skin elasticity.Conclusion Schisandra polysaccharide ointment has a protective effect on the skin of rabbits.This study provides a theoretical basis for the development of skin care products.