三种根管封闭剂根尖封闭性能的体外研究

Study on the apical sealing property for three kinds of root canal sealers in vitro

目的在体外环境下比较HiFlow、iRoot SP、AH Plus 3种根管封闭剂的根尖封闭性能,以评价新型生物陶瓷类根管封闭剂HiFlow的应用效果。方法选取46个单根前磨牙,随机分为3个实验组(n=12)和一个对照组(n=10),实验组分别用HiFlow封闭剂、iRoot SP封闭剂、AH Plus封闭剂配合牙胶尖行单尖法充填;对照组只用牙胶尖行单尖法充填。每组随机选取10个样本,使用染料渗透法对样本处理后,在体视显微镜下测量染料渗透长度,比较各组染料渗透长度。测量并比较不同组根尖微渗漏水平。剩余的实验组样本横切后用扫描电子显微镜观察截面。结果HiFlow组、iRoot SP组、AH Plus组和对照组的根管内均有染料渗入,染料渗透长度依次为(1.027±0.04)、(1.079±0.05)、(1.132±0.12)和(2.46±0.75)mm。3个实验组之间的染料渗透长度差异均无统计学意义(P>0.05),实验组染料渗透长度均明显小于对照组(P<0.05)。通过扫描电镜观察到3组根管封闭剂与根管壁牙本质之间均有间隙,AH Plus组的缝隙最大,HiFlow组的缝隙最小。结论3种根管封闭剂均不能完全封闭根管,其中HiFlow根尖封闭性能相对最佳。

Objective To evaluate the effect of HiFlow,a new bioceramic root canal sealer,by comparing the apical sealing properties of HiFlow、iRoot SP and AH-Plus root canal sealers in vitro.Methods 46 singlerooted premolars were selected and randomly divided into 3 experimental groups(n=12)and a control group(n=12).The experimental group was filled with HiFlow sealer,iRoot SP sealer,AH Plus sealer in combination with gum tips using single cone obturation technique;the control group was filled with a single cone obturation technique.10 samples from each group were selected and treated using the dye penetration method.The length of dye penetration was measured under a stereomicroscope to compare the length of dye penetration in each group.The level of microleakage at the root apical level was measured and compared between the different groups.The remaining samples from the experimental group were cross-sectioned and observed in profile with a scanning electron microscopy.Results Dye infiltration was observed in the root canals of the HiFlow group,iRoot SP group,AH Plus group,and control group,with dye infiltration lengths in the order of(1.027±0.04)mm,(1.079±0.05)mm,(1.132±0.12)mm,and(2.46±0.75)mm,respectively.The difference in dye penetration length among the three experimental groups was not statistically significant(P>0.05),and the dye penetration length in the experimental groups were all significantly smaller than that in the control group(P<0.05).Through scanning electron microscopy,it was observed that there were gaps between the root canal sealers and the dentin of the root canal wall in all three experimental groups,with the largest gap in the AH Plus group and the smallest gap in the HiFlow group.Conclusion None of the three kinds of root canal sealers could completely seal the root canal,while HiFlow had the best apical sealing performance.