基于数据挖掘分析UHRF1在肺腺癌中的表达及临床意义

Expression of UHRF1 in lung adenocarcinoma by data mining and its clinical significance

目的:旨在基于生物信息数据库进行数据挖掘以探索泛素样含PHD和环指域1(UHRF1)在肺腺癌中的表达情况并分析其对肺腺癌患者的预后影响。方法:使用GEPIA数据库探索UHRF1在泛癌中的表达情况。应用UALCAN数据库和Kaplan-Meier Plotter数据库分析UHRF1和肺腺癌患者的临床相关性及预后的意义。通过GSEA分析UHRF1的相关功能。在String数据库中分析与UHRF1相互作用的蛋白。运用TCIA数据库分析肺腺癌中UHRF1表达水平和免疫浸润的关系。结果:UHRF1在大部分癌症存在表达差异,肺腺癌组织中UHRF1表达水平和正常组织相比显著升高(P<0.05)。UHRF1表达量和肺癌患者的年龄(P<0.05)、性别(P<0.05)、吸烟习惯(P<0.05)、病理分期(P<0.05)显著相关。Kaplan-Meier曲线表明UHRF1高表达和不良预后相关(P<0.05)。GSEA、蛋白-蛋白相互作用和免疫浸润分析表明UHRF1可能作为一个潜在的分子生物标志物,通过影响肺腺癌细胞的增殖、迁移、DNA甲基化和肿瘤免疫浸润,从多个方面促进肺腺癌的发生发展。结论:UHRF1可能作为肺腺癌预后的生物标志物,具有重要的临床价值。

Objective To explore the expression of UHRF1(Ubiquitin⁃like with PHD and RING finger domain 1)in lung adenocarcinoma and analyze its prognostic impact on lung adenocarcinoma patients by conducting data mining based on bioinformatic databases.Methods The expression of UHRF1 in pan⁃cancer was explored using the GEPIA database.The UALCAN and Kaplan⁃Meier Plotter database were used to analyze the clinical correlation and prognostic significance of UHRF1 in lung adenocarcinoma patients.The related functions of UHRF1 were analyzed by GSEA.Proteins interacting with UHRF1 were analyzed in the String database.The relationship between the level of UHRF1 expression and immune infiltration in lung adenocarcinoma was analyzed using the TCIA database.Results The expression of UHRF1 was different in pan⁃cancers,and the expression level of UHRF1 in lung adenocarcinoma tissues was significantly higher than that in normal tissues(P<0.05).The expression of UHRF1 was significantly correlated with age,gender,smoking habits,and pathological stages of lung cancer patients.Kaplan⁃Meier curves indicated that high UHRF1 expression was associated with poor prognosis(P<0.05).GSEA,protein⁃protein interaction(PPI)and immune infiltration analysis suggest that UHRF1 may serve as a potential molecular biomarker and promote lung adenocarcinoma by DNA methylation,ubiquitination,proliferation,migration and tumor immune infiltration.Conclusion UHRF1 may serve as a prognostic biomarker for lung adenocarcinoma and has important clinical significance.