香豆素荧光探针在生物硫醇检测中的研究进展

Research Progress in Coumarin Fluorescent Probes for Biothiols Detection

生物硫醇在动植物细胞中发挥着重要作用,其水平的变化与生物体的一些重大疾病相关,因此,快速、灵敏、准确地检测生物样品中生物硫醇非常重要。荧光成像分析法由于灵敏度高、时空分辨率高、原位实时等优点,已被广泛应用于生物硫醇的检测。基于香豆素荧光探针与生物硫醇的相互作用模式对香豆素荧光探针进行了分类,综述了各类香豆素荧光探针的设计、检测机理及其在活细胞成像中的应用研究进展,并对香豆素荧光探针在生物硫醇检测领域的应用前景进行了展望。

Biothiols play an important role in plant and animal cells,and the change in their levels is related to some major biological diseases.Therefore,it is very important to quickly,sensitively,and accurately detect biothiols in biological samples.Fluorescence imaging analysis has been widely used for the detection of biothiols due to its advantages of high sensitivity,high spatiotemporal resolution,and in situ real-time.In this paper,we classify the coumarin fluorescent probes based on the interaction modes between coumarin probes and biothiols.Moreover,we review the design,detection mechanism,and application research progress of various coumarin fluorescent probes in living cell imaging.Furthermore,we put forward the application prospects of coumarin fluorescent probes in the field of biothiols detection.