完全基于质粒的轮状病毒反向遗传技术进展

Advances in Reverse Genetic Technology for Rotavirus Based Entirely on Plasmids

人轮状病毒(Rotavirus,RV)为双链RNA病毒,属于呼肠孤病毒科轮状病毒属,是全球5岁以下婴幼儿重症腹泻的主要病因。2017年,完全基于质粒的轮状病毒反向遗传技术获得成功,此后被不断改进。该技术可成功获得体外人工改造的特定表型的轮状病毒,促进和丰富了轮状病毒感染致病机制研究以及新一代轮状病毒疫苗、药物等干预手段的开发。本文将概述和比较已报道的不同轮状病毒反向遗传技术特征,以期推动我国轮状病毒反向遗传技术体系的建立,促进我国轮状病毒的科学研究及预防控制。

Human rotavirus(RV)is a double⁃stranded RNA virus,belonging to the genus rotavirus in the family Reoviridae.It is the main cause of severe diarrhea in infants and young children under 5 years of age worldwide.In 2017,the reverse genetic technology of rotavirus,which is entirely plasmid⁃based,was successful and has been continuously improved since then.This technology can be used to obtain artificially modified specific phenotypes of rotavirus in vitro,which promotes and enriches the research on the pathogenic mechanism of rotavirus infection,and development of new generation of rotavirus vaccines,drugs and other interventions.In this paper,we will summarize and compare the reported characteristics of different rotavirus reverse genetic technologies,in order to promote the establishment of rotavirus reverse genetic system in China,and promote the scientific research,prevention and control of rotavirus in China.