吲哚菁绿荧光显影联合亚甲蓝染色在乳腺癌前哨淋巴结活检中的应用

Application of indocyanine green fluorescence imaging combined with methylene blue staining in sentinel lymph node biopsy of breast cancer

目的:探讨吲哚菁绿(ICG)和亚甲蓝(MB)联合应用于早期乳腺癌前哨淋巴结活检(SLNB)的可行性。方法:回顾性分析2014年1月至2023年9月415例接受SLNB和腋窝淋巴结清扫术(ALND)的患者资料。根据不同染色方法分为MB+ICG组(n=197)和MB组(n=218)。数据分析用SPSS 26.0软件进行。SLNB检出率及假阴性率检验比较采用χ^(2)检验,必要时进行Bonferroni校正。P<0.05差异有统计学意义。结果:MB+ICG组SLN的检出率为96.9%,显著高于MB组的89.7%(P<0.05)。同样,联合组平均每例SLN数为3.0枚,明显高于MB组的2.1枚(P<0.05)。联合组和单用MB组的假阴性率分别为7.3%和10.5%,差异无统计学意义(P=0.791)。结论:ICG和MB联合应用于SLNB较单独应用MB更能有效检测SLN。

Objective:To investigate the feasibility of combined application of indocyanine green(ICG)and methylene blue(MB)in sentinel lymph node biopsy(SLNB)of early breast cancer.Methods:Data of 415 patients who underwent SLNB and axillary lymph node dissection from January 2014 to September 2023 were retrospectively analyzed.They were divided into MB+ICG group(n=197)and MB group(n=218)according to different staining methods.SPSS 26.0 software was used for data analysis.The detection rate and false negative rate of SLNB were compared byχ^(2) test,and Bonferroni correction was performed if necessary.P<0.05 was statistically significant.Results:The detection rate of SLN in MB+ICG group was 96.9%,which was significantly higher than 89.7%in MB group(P<0.05).Similarly,the average SLN number per patient in the combined group was 3.0,which was significantly higher than 2.1 in the MB group(P<0.05).The false negative rates of the combined group and the MB group were 7.3%and 10.5%,respectively,with no significant difference(P=0.791).Conclusion:ICG and MB combined in SLNB can detect SLN more effectively than MB alone.