刺山柑果实的化学成分分离与鉴定

Isolation and Identification of Chemical Constituents from the Fruits of Capparis spinosa

目的:研究山柑科山柑属植物刺山柑Capparis spinosa L.果实中的化学成分。方法:利用硅胶柱、Sephadex LH-20葡聚糖凝胶柱、半制备高效液相等色谱方法对刺山柑果实进行分离,根据理化性质和波谱数据进行结构鉴定。结果:从刺山柑果实80%乙醇提取物的乙酸乙酯及二氯甲烷萃取部位中分离得到15个化合物,分别鉴定为:2-(furan-2-yl)-5-(2,3,4-trihydroxybutyl)-1,4-diazine(1)、3,4-dihydroxyquinoline-4-O-β-D-glucopyranoside(2)、二氢菜豆酸(3)、表二氢菜豆酸(4)、吐叶醇(5)、异香草酸(6)、覆盆子酮(7)、(S)-5-[(R)-1-hydroxyhexyl]-4,5-dihydrofuran-2(3H)-one(8)、chaenomester C(9)、2-甲基苯并恶唑-7-羧酸(10)、反式肉桂酸(11)、对羟基苯甲酸(12)、吲哚乙酸(13)、香草酸(14)、芦丁(15)。结论:其中,化合物10为新的天然产物,化合物1~9为首次从该属植物中分离得到。

Objective:To study the chemical constituents from the fruits of Capparis spinosa.Methods:The fruits of Capparis spinosa were isolated by silica gel column chromatography,Sephadex LH-20 column chromatography,semi preparative high performance liquid chromatography and other methods,the structures were identified by physical and chemical properties and spectral data.Results:Fifteen compounds were isolated from ethyl acetate and dichloromethane extract of 80%ethanol extract of Capparis spinosa fruits and identified as 2-(furan-2-yl)-5-(2,3,4-trihydroxybutyl)-1,4-diazine(1),3,4-dihydroxyquinoline-4-O-β-D-glucopyranoside(2),dihydrophaseic acid(3),epidihydrophaseic acid(4),vomitol(5),3-hydroxy-4-methoxybenzoic acid(6),4-(4-hydroxyphenyl)-2-butanone(7),(S)-5-[(R)-1-hydroxyhexyl]-4,5-dihydrofuran-2(3H)-one(8),chaenomester C(9),2-methyl-1,3-benzoxazole-7-carboxylic acid(10),trans-cinnamic acid(11),4-hydroxybenzoic acid(12),indole-3-acetic acid(13),vanillic acid(14),rutin(15),respectively.Conclusion:Among them,compound 10 is a new natural product,compounds 1 to 9 are isolated from Capparis genus for the first time.