复方一枝蒿颗粒体外抗呼吸道合胞病毒的作用及对先天免疫信号通路的影响

Anti-respiratory syncytial virus effects and mechanism on the innate immune signaling pathway of Compound Yizhihao granules in vitro

目的研究复方一枝蒿颗粒体外抗呼吸道合胞病毒(RSV)的作用及对病毒诱导激活先天免疫信号通路的影响。方法Viral Tox Glo法测定复方一枝蒿颗粒体外抗呼吸道合胞病毒的作用;Western Blotting法测定先天免疫通路蛋白表达的水平;流式微球技术测定细胞因子表达的水平。结果复方一枝蒿颗粒在体外对呼吸道合胞病毒有明显的抑制作用,且药物浓度与抗病毒作用呈量效关系;复方一枝蒿颗粒可促进先天免疫信号通路靶点核因子κB(NF-κB)抑制蛋白α、NF-κBp65蛋白的表达水平,降低TANK结合激酶1(TBK1)、丝氨酸/苏氨酸激酶的表达水平,对RSV病毒诱导表达的炎症因子白介素-2(IL-2)、IL-6、IL-10和肿瘤坏死因子-α有明显的干预作用,表现出下调趋势。结论复方一枝蒿颗粒可通过作用于先天免疫信号通路TBK1/NF-κB发挥免疫调节作用,从而达到抗呼吸道合胞病毒的作用。

OBJECTIVE To study anti-respiratory syncytial virus(RSV)effects and the effect on virus-induced activation of the innate immune signaling pathway of Compound Yizhihao granules(CYG)in vitro.METHODS Viral ToxClo method was used to evaluate the anti-RSV effect of CYG in vitro.Western Blotting was used to detect the expression levels of innate immune pathway proteins.Flow microsphere technology was applied to detect cytokine expression levels.RESULTS CYG showed inhibitory effect on RSV in vitro,and there was a dose dependent-effect between drug concentration and antiviral effect.Further experiments found that CYG could promotes the protein expression levels of inhibitor κBα and NF-κB p65,and reduce the levels of TANK-binding kinase 1(TBKI)and protein kinase B.And CYG had significantly interfered the expression levels of IL-2,IL-6,IL-10 and TNF-αinduced by RSV,showing a down-regulation trend.CONCLUSION The CYG can exert immunomodulatory effects by acting on the innate immune signaling pathway TBK1/NF-κB,thus achieving anti-RSV effects.