灰色关联度分析清半夏体外抗氧化作用的谱效关系

Grey correlation analysis of the spectrum effect relationship of in vitro antioxidant effec of Pinellia Rhizoma praeparatum cum alumine

目的通过灰色关联度分析12批清半夏与其体外抗氧化作用间的谱效关系,寻找其体外抗氧化作用的物质基础。方法建立清半夏的HPLC指纹图谱,以DPPH、ABTS自由基清除率及总还原能力的半抑制浓度为药效指标,采用灰色关联度分析其谱效关系。结果建立的指纹图谱中确定了7个共有峰,并指认了6个,相似度均>0.977。聚类分析将12批清半夏饮片分为5类。对12批清半夏与体外抗氧化作用进行谱效关联,其关联度均>0.6,表明其体外抗氧化作用是由多种成分共同作用,对其贡献较大的为峰1(草酸)、峰6(鸟苷)、峰3(腺嘌呤)。结论清半夏体外抗氧化作用的主要成分为草酸、鸟苷、腺嘌呤,为清半夏抗氧化作用机制的深入研究提供了依据。

OBJECTIVE To analyze the crrelation between 12 batches of Pinellia Rhizoma praeparatum cum alumine(PRPCA)and its antioxidant activity in vitro by grey correlation to find the material basis of its antioxidant activity.METHODS The HPLC fingerprint of PRPCA was established,and the ICso values of DPPH,ABTS free radical scavenging rate and total reducing power were used as pharmacodynamic indexes.RESULTS The established fingerprint identified 7 common peaks and 6 peaks,and the similarity was greater than 0.977.The 12 batches of PRPCA decoction were divided into 5 categories by cluster analysis.The spectral correlation between 12 batches of PRPCA and the antioxidant effect in vitro was greater than O.6,indicating that the antioxidant effect in vitro was caused by the combined action of various components,and the major contributions were peak 1(oxalic acid),peak 6(guanosine)and peak 3(adenine).CONCLUSION The main components of antioxidation in vitro may be oxalic acid,guanosine and adenine,which provides scientific basis for further study of antioxidation mechanism of PRPCA.