蜂王浆水溶蛋白对高糖诱导人脐静脉内皮细胞的保护作用

Protective effects of water-soluble royal jelly protein on high glucose induced human umbilical vein endothelial cells

目的研究蜂王浆水溶蛋白对高糖诱导人脐静脉内皮细胞(HUVEC)的保护作用及其机制。方法采用CCK-8法检测蜂王浆水溶蛋白对HUVEC细胞增殖活性的影响;酶标法检测蛋白对超氧化物歧化酶(SOD)分泌的影响;酶联免疫吸附法检测蛋白对肿瘤坏死因子α(TNF-α)、白介素-4(IL-4)分泌的影响;流式细胞术检测蛋白对活性氧分泌的影响;Hoechst-PI双染法检测蛋白对细胞凋亡及坏死的影响。结果高糖损伤的HUVEC经蜂王浆水溶蛋白处理后,细胞的形态明显改善,增殖活性提高,SOD分泌增加,清除活性氧的能力提高,TNF-α、IL-4的分泌减少,细胞凋亡减少,但对细胞坏死率无影响。结论蜂王浆水溶蛋白能抗高糖所致HUVEC的损伤,其机制可能与提高细胞抗氧化能力、减轻炎症反应有关。

OBJECTIVE To study the protective effect and mechanism of water-soluble royal jelly protein(WSRJP)on high glucose induced human umbilical vein endothelial cells(HUVEC).METHODSSCCK-8 method was used to detect the effect of WSRJP on the proliferation activity of HUVEC.Enzyme labeling method was used to detect the effect of WSRJP on the secretion of superoxide dismutase(SOD).Enzyme-linked immunosorbent assay was used to detect the effect of WSRJP on the secretion of inflammatory factors tumor necrosis factorα(TNF-α)and interleukin-4(IL-4).Flow cytometry was used to detect the effect of WSRJP on the secretion of reactive oxygen species.Hoechst-PI double staining method was used to detect the effect of WSRJP on apoptosis and cell necrosis.RESULTS After treatment with WSRJP,HUVEC damaged by high glucose showed significant improvement in cell morphology and increased proliferation activity,and the reactive oxygen species secretion was increased.The ability to clear reactive oxygen species was improved,and the secretion of TNF-αand IL-4 was decreased.Apoptosis is reduced,but there is no effect on cell necrosis rate.CONCLUSIONWSRJP can resist high glucose induced HUVEC damage,and its mechanism may be related to improving cell antioxidant capacity and reducing inflammatory response.