表儿茶素没食子酸酯与菊粉复配对鲈鱼肌球蛋白的影响

Effect of(-)-Epicatechin Gallate and Inulin Complex on Myosin from Lateolabrax japonicus

以鲈鱼肌球蛋白为研究对象,通过分析浊度、粒径、电位、溶解度、活性巯基、二聚酪氨酸含量等,探究不同质量分数(0.1%,0.2%,0.3%,0.4%)的表儿茶素没食子酸酯复配多糖(1%的菊粉)对鲈鱼肌球蛋白特性的影响。结果表明,随着表儿茶素没食子酸酯质量分数的增加,浊度与粒径呈先下降后上升的趋势,电位、溶解度、活性巯基含量呈先上升后降低的趋势,而二聚酪氨酸的变化趋势则与之相反。添加0.2%表儿茶素没食子酸酯与1%菊粉的鲈鱼肌球蛋白,浊度与粒径最小,粒径降低到279 nm;电位绝对值、溶解度以及活性巯基值最大,电位绝对值以及活性巯基分别为6.37 mV,24.34 nmol/mg prot;二聚酪氨酸含量最低。因此,不同质量分数的表儿茶素没食子酸酯与1%的菊粉协同作用可改善鲈鱼肌球蛋白的理化性质,研究结果为多酚协同多糖改善鱼糜凝胶特性提供了理论依据。二聚酪氨酸的含量表明,多酚协同多糖还可以增加肌球蛋白的抗氧化性。

In order to investigate the effect of(-)-epicatechin gallate(0.1%,0.2%,0.3%,0.4%)synergized with polysaccharide(1%inulin)on the characteristics of myosin,turbidity,particle size,zeta potential,solubility,active sulfhydryl group and dimeric tyrosine content were analyzed.The results showed that with the increase of mass fraction of(-)-epicatechin gallate,the turbidity and particle size showed a trend of decreasing and then increasing,while the zeta potential,solubility and active sulfhydryl groups content increased first and then decreased,the change trend of dimeric tyrosine was opposite.When 0.2%(-)-epicatechin gallate and 1%inulin were added,the turbidity and particle size were the smallest,and the particle size was reduced to 279 nm.The absolute values of potential,solubility,and active sulfhydryl group values were the hightest when 0.2%(-)-epicatechin gallate and 1%inulin were added.The absolute potential value and active sulfhydryl group were respectively 6.37 mV,24.34 nmol/mg prot.The dimeric tyrosine contents were the lowest.Therefore,the synergistic effect of different concentrations of(-)-epicatechin gallate and 1%of inulin improved the physicochemical properties of myosin in Lateolabrax japonicus,providing a theoretical basis for the improvement of surimi gel properties,while the dimeric tyrosine indicated that it also increased the antioxidant properties of myosin.