大豆FCS-like Zinc Finger家族基因的生物信息学鉴定

Bioinformatics Identification of Soybean FCS-like Zinc Finger Gene Family

FCS-like Zinc Finger (FLZ)是一类植物特有的基因家族,在植物生长发育以及对逆境胁迫的响应中有着重要的调控作用。然而,目前对于大豆FLZ家族基因特征和功能的研究仍然缺乏。本研究利用Hmmsearch方法从大豆基因组中鉴定了40个FLZ (GmFLZs)基因,并分析了GmFLZ基因家族的蛋白理化性质、基因结构、启动子顺式作用元件、进化和基因表达等特征。结果表明,Gm FLZ蛋白平均氨基酸数目为220.7,均为亲水性蛋白。进化分析表明Gm FLZ蛋白可分为7个亚组。GmFLZ基因启动子序列中有丰富的与光信号、激素和胁迫响应相关的顺式作用元件。GmFLZ家族基因分布于20条染色体上,之间未发现串联重复,而有28个基因参与片段重复。共线性分析表明15个GmFLZ基因同时与绿豆和拟南芥FLZ基因间存在同源关系。Ka/Ks分析结果表明GmFLZ基因在进化过程中经历了不同的进化方向。蛋白质Docking模拟结果表明GmFLZ7和GmFLZ9可与SnRK1α亚基对接。本研究结果为阐明大豆FLZ家族基因的功能提供了参考。

The FCS-like Zinc Finger(FLZ) represents one of the plant-specific gene family, which plays an important regulatory role in plant growth and development and response to adversity stress. However, less is known about the characteristics and functions of FLZ genes in Glycine max. In this study, a total of 40 FLZ(GmFLZs) genes were identified from the G. max genome using the Hmmsearch method, and the protein physicochemical properties,gene structure, promoter cis-acting elements, evolution and gene expression characteristics of GmFLZs were analyzed. The results showed that the average number of amino acids of Gm FLZ proteins was 220.7, all of which were hydrophilic proteins. Phylogenetic analysis reveals that Gm FLZs can be divided into 7 clusters. There are abundant cis-acting elements related to light signal, hormone and stress response in the promoter sequence of GmFLZs. The GmFLZs are distributed on 20 chromosomes of G. max, and the tandem duplication is not identified,while 28 GmFLZs are involved in segmental duplications. Collinearity analysis showed that 15 GmFLZs were homologous to the FLZ genes in Vigna radiata and Arabidopsis thaliana. Ka/Ks analysis results show that the GmFLZs have experienced different evolutionary directions during the evolution process. The results of protein docking simulation show that GmFLZ7 and GmFLZ9 can dock with the SnRK1α subunit. The results of this study serve as a reference for elucidating the functions of FLZ family genes in G. max.